Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all YM1/Chitinase 3-like 3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3
Tyr22-Tyr398
Accession # O35744

Specificity

Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse YM1/Chitinase 3-like 3 Antibody

Detection of Mouse YM1/Chitinase 3-like 3 antibody by Western Blot.

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse YM1/Chitinase 3-like 3 antibody by Simple WesternTM.

Detection of Mouse YM1/Chitinase 3‑like 3 by Simple WesternTM.

Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3-like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of YM1/Chitinase 3-like 3 by Western Blot

Detection of YM1/Chitinase 3-like 3 by Western Blot

TRPV1 suppresses the expression of Arg1 and Ym1 in activated microglia. (A,B) Quantitative PCR (qPCR) analysis of Chil3(A) and Arg1(B) mRNA expression in cortical tissues of WT (n = 6) and TRPV1−/−(n = 6) mice after rFS. Values were shown for steady-state transcripts relative to beta -actin. Data were presented as mean ± SEM (n = 6 per groups, *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons). (C–E) Representative Immunoblot bands (C) and densitometric analysis of Arg1 expression (D) and Ym1 expression (E) were significantly decreased in rFS from WT mice than in TRPV1−/− mice (n = 6 per groups, *p < 0.05, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons). (F–H) Representative Immunoblot bands (F) and densitometric analysis of Arg1 expression (G) and Ym1 expression (H) was significantly decreased in the cortex microglia of WT mice than in TRPV1−/− mice after treatment of Capsaicin (10 μM), Hyperthermia (43°C, 4*30 min) and LPS (1.0 μg/ml), respectively. Data were presented as means ± SEM, n = 6 per groups, *p < 0.05, **p < 0.01, two-way ANOVA followed by Tukey’s multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31680864), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse YM1/Chitinase 3-like 3 Antibody

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: Mouse bone marrow

Western Blot

0.2 µg/mL
Sample: Mouse bone marrow

Reviewed Applications

Read 1 review rated 5 using AF2446 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YM1/Chitinase 3-like 3

YM1, also known as Chitinase 3-like 3 and ECF-L, is a secreted 377 amino acid residue lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. YM1 shares homology with chitinase family proteins but lacks enzymatic activity.

Alternate Names

CHI3L3, Chitinase 3 like 3, Chitinase 3-like 3, ECF-L

Entrez Gene IDs

12655 (Mouse)

Gene Symbol

CHIL3

UniProt

O35744

Additional YM1/Chitinase 3-like 3 Products

Product Documents for Mouse YM1/Chitinase 3-like 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse YM1/Chitinase 3-like 3 Antibody

For research use only

Citations for Mouse YM1/Chitinase 3-like 3 Antibody

Customer Reviews for Mouse YM1/Chitinase 3-like 3 Antibody (1)

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  • Mouse YM1/Chitinase 3-like 3 Antibody
    Name: Branislav Krljanac
    Application: Immunohistochemistry-Frozen
    Sample Tested: Nippostrongylus brasiliensis infected mouse lung (day 14 post infection)
    Species: Mouse
    Verified Customer | Posted 07/16/2017
    Nippostrongylus brasiliensis infected mouse lung, day 14 post infection. Goat-Ym-1 (AF2446) 1/100 in PBS+2,5% BSA (1h) Donkey-anti-Goat-A488 1/150 in 2,5% donkey serum (1h) Mounting media containing DAPI.
    Nippostrongylus brasiliensis infected mouse lung (day 14 post infection) Frozen section (acetone fixed) Blocking in 2,5% donkey serum (30min) Goat-Ym-1 (AF2446) 1/100 in PBS+2,5% BSA (1h) Donkey-anti-Goat-A488 1/150 in 2,5% donkey serum (1h) Mounting media containing DAPI.
    Mouse YM1/Chitinase 3-like 3 Antibody AF2446

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