Notch-1 Antibody (mN1A) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80884

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat (Negative)

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # mN1A

Format

Azide and BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse Notch1 protein [Uniprot: Q01705]

Reactivity Notes

Does not recognize rat Notch1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

272.505 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-1 Antibody (mN1A) - Azide and BSA Free

Western Blot: Notch-1 Antibody (mN1A)Azide and BSA Free [NBP2-80884]

Western Blot: Notch-1 Antibody (mN1A)Azide and BSA Free [NBP2-80884]

Western Blot: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). Image from the standard format of this antibody.
Immunohistochemistry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Immunohistochemistry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Immunohistochemistry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous p
Flow Cytometry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Flow Cytometry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Flow Cytometry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Knockout Validated: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Western Blot: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884]

Western Blot: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

Applications for Notch-1 Antibody (mN1A) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:100

Western Blot

1 - 2 ug/ml
Application Notes
The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Notch-1

Notch1, also known as neurogenic locus notch homolog protein 1, is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer - in particular certain leukemias.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (mN1A) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Notch-1 Antibody (mN1A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody (mN1A) - Azide and BSA Free

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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