Notch-1 Antibody (mN1A) - BSA Free

Novus Biologicals | Catalog # NB100-78486

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # mN1A

Format

BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse Notch1 protein [Uniprot: Q01705]

Reactivity Notes

Does not recognize rat Notch1.

Specificity

Does not cross-react with Notch 2, 3, or 4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

272.505 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-1 Antibody (mN1A) - BSA Free

Knockout Validated: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Western Blot: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Notch-1 Antibody (mN1A)BSA Free [NB100-78486]

Western Blot: Notch-1 Antibody (mN1A)BSA Free [NB100-78486]

Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD).
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).
Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.
Flow Cytometry: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Flow Cytometry: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486]

Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Notch-1 Antibody (mN1A) - BSA Free

Western Blot: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486] -

The DA-MeHA hydrogel regulated Notch signaling.A–E DA-MeHA hydrogel-encapsulated spheroids or suspensions of ADSCs upregulated the expression of Notch1 and Notch2 while downregulating the expression of Notch3, Jagged1 and Jagged2. F No significant difference in Hes1 expression was observed among the groups. n = 4 samples/group (*p < 0.05 and **p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36030275), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Notch-1 Antibody (mN1A) - BSA Free

Western Blot: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486] -

Interleukin-33 (IL-33) decreases bone marrow-derived monocytes (BMMs) osteoclastogenesis by inhibiting Notch1 activity. (A) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (B–D) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (E) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors induced by IL-33 in the presence of miR-34a-5p antagomir. (F–H) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors treated with IL-33 in the presence of miR-34a-5p antagomir. (I–K) BMMs osteoclastic formation and tartrate-resistant acid phosphatase (TRAP) activity assay after treatment with 20 ng/ml IL-33 in the presence of Jag-1. Red arrows indicated multinucleated osteoclasts. Scale bars represent 100 um. (L–O) Quantification of Trap, Cathepsin K, Nfatc1, and C-fos expression in osteoclast progenitors treated with 20 ng/ml IL-33 in the presence of Jag-1. *P < 0.05, **P < 0.01, ***P < 0.001. P values were analyzed by one-way ANOVA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29085370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Notch-1 Antibody (mN1A) - BSA Free

Western Blot: Notch-1 Antibody (mN1A) - BSA Free [NB100-78486] -

Interleukin-33 (IL-33) decreases bone marrow-derived monocytes (BMMs) osteoclastogenesis by inhibiting Notch1 activity. (A) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (B–D) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (E) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors induced by IL-33 in the presence of miR-34a-5p antagomir. (F–H) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors treated with IL-33 in the presence of miR-34a-5p antagomir. (I–K) BMMs osteoclastic formation and tartrate-resistant acid phosphatase (TRAP) activity assay after treatment with 20 ng/ml IL-33 in the presence of Jag-1. Red arrows indicated multinucleated osteoclasts. Scale bars represent 100 um. (L–O) Quantification of Trap, Cathepsin K, Nfatc1, and C-fos expression in osteoclast progenitors treated with 20 ng/ml IL-33 in the presence of Jag-1. *P < 0.05, **P < 0.01, ***P < 0.001. P values were analyzed by one-way ANOVA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29085370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Notch-1 Antibody (mN1A) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:100

Western Blot

1 - 2 ug/mL
Application Notes
The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms.

This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NB100-78486 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Notch-1

Notch1, also known as neurogenic locus notch homolog protein 1, is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer - in particular certain leukemias.

Alternate Names

Notch1, TAN1

Entrez Gene IDs

4851 (Human); 18128 (Mouse)

Gene Symbol

NOTCH1

UniProt

Q01705

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (mN1A) - BSA Free

Certificate of Analysis

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Product Specific Notices for Notch-1 Antibody (mN1A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Notch-1 Antibody (mN1A) - BSA Free

Customer Reviews for Notch-1 Antibody (mN1A) - BSA Free (2)

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Customer Images

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  • Notch-1 Antibody (mN1A)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Vascular Smooth Muscle Cells
    Species: Mouse
    Verified Customer | Posted 12/07/2021
    Excellent Notch-1 antibody
    Notch-1 Antibody (mN1A) - BSA Free NB100-78486
  • Name: Shyam Gudey
    Application: Western Blot
    Sample Tested:
    Species: Other
    Verified Customer | Posted 01/10/2014

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FAQs for Notch-1 Antibody (mN1A) - BSA Free

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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