Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Novus Biologicals | Catalog # NB300-251

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Format

BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)

Specificity

This antiserum is directed against human Notch-1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat Notch-1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This antiserum is directed against human Notch-1

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.
Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251]

Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251]

Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.
Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody(Cleaved N terminal) [NB300-251]

Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
Notch-1 Antibody - (Cleaved N terminal)

Notch-1 Antibody - (Cleaved N terminal)

Immunohistochemistry of Rabbit Notch-1 Antibody - (Cleaved N terminal). Tissue: Exocrine glands of human pancreas. Fixation: FFPE. Primary antibody: Notch1 antibody at 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
Notch-1 Antibody - (Cleaved N terminal)

Notch-1 Antibody - (Cleaved N terminal)

Dot Blot of Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody. Antigen: Row 1 - Notch 1 Peptide (Cleaved N Terminal) Row 2 - Notch 1 (Intra) Peptide. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: Rabbit anti-Notch-1 Antibody - (Cleaved N terminal)at 1:1,000 for 60 min at RT. Secondary antibody: HRP Rabbit Secondary at 1:40,000 for 30 min at RT. Block for 1 HR at RT.

Applications for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:60000

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500-1:2000
Application Notes
This product has been tested by ELISA, dot blot, western blot and immunohistochemistry. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. Specific conditions for reactivity should be optimized by the end user.

Reviewed Applications

Read 1 review rated 1 using NB300-251 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Entrez Gene IDs

4851 (Human); 18128 (Mouse); 25496 (Rat)

Gene Symbol

NOTCH1

UniProt

P46531

Additional Notch-1 Products

Product Documents for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Customer Reviews for Notch-1 Antibody - (Cleaved N terminal) - BSA Free (1)

1 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
100%

Have you used Notch-1 Antibody - (Cleaved N terminal) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Notch-1 Antibody
    Name: Ram Kuwar
    Application: Western Blot
    Sample Tested: Rat brain tissue
    Species: Rat
    Verified Customer | Posted 10/23/2020
    The bands appeared in 20KD.
    Notch-1 Antibody - (Cleaved N terminal) - BSA Free NB300-251
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody - (Cleaved N terminal) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies