Phospho-GSK-3 beta (S9) DuoSet IC ELISA

Discontinued Product

DYC1590-2 has been discontinued.
View all GSK-3 beta products.
R&D Systems DuoSet ELISAs
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Product Details
Citations (5)

Phospho-GSK-3 beta (S9) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
312.0 - 20,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GSK-3 beta

GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.

Long Name:
Glycogen Synthase Kinase 3 beta
Entrez Gene IDs:
2932 (Human); 56637 (Mouse); 84027 (Rat)
Alternate Names:
EC 2.7.11; EC; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Citations for Phospho-GSK-3 beta (S9) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IL-13 orchestrates resolution of chronic intestinal inflammation via phosphorylation of glycogen synthase kinase-3beta.
    Authors: Fichtner-Feigl S, Kesselring R, Martin M, Obermeier F, Ruemmele P, Kitani A, Brunner S, Haimerl M, Geissler E, Strober W, Schlitt H
    J Immunol, 2014-03-14;192(8):3969-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Real-time monitoring of Cisplatin-induced cell death.
    Authors: Alborzinia H, Can S, Holenya P, Scholl C, Lederer E, Kitanovic I, Wolfl S
    PLoS ONE, 2011-05-16;6(5):e19714.
    Species: Human
    Sample Types: Cell Lysates
  3. Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis.
    Authors: Holenya P, Kitanovic I, Heigwer F, Wolfl S
    Proteomics, 2011-04-18;11(10):2129-33.
    Species: Human
    Sample Types: Recombinant Protein
  4. Canonical Wnt signaling negatively regulates platelet function.
    Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
    Proc. Natl. Acad. Sci. U.S.A., 2009-11-09;106(47):19836-41.
    Species: Human
    Sample Types: Cell Lysates
  5. Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
    Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
    J. Immunol., 2009-01-01;182(1):547-53.
    Species: Human
    Sample Types: Cell Lysates


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