Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Porcine IFN-alpha Antibody (F17)
R&D Systems | Catalog # 27105-1
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Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Porcine
Applications
Validated:
Neutralization
Cited:
Flow Cytometry, ELISA Detection, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # F17
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Product Specifications
Immunogen
Porcine IFN-alpha
Specificity
Neutralizes pig interferon alpha
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Applications for Porcine IFN-alpha Antibody (F17)
Application
Recommended Usage
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using 27105-1 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied as a liquid
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, this product should be kept at -20°C for retention of full activity. Do not store below -20°C.
Background: IFN-alpha
References
- L'Haridon, R.M. et al. 1991, Hybridoma 10: 35-47.
- Splichal, I. et al. 1994, Immunology Letters 43:203-208.
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Porcine IFN-alpha Antibody (F17)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IFN-alpha Antibody (F17)
For research use only
Citations for Porcine IFN-alpha Antibody (F17)
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Customer Reviews for Porcine IFN-alpha Antibody (F17) (2)
4.5 out of 5
2 Customer Ratings
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Application: Immunohistochemistry-FrozenSample Tested: spleen, Thymus tissue, Placental tissue and Lymph nodeSpecies: PigVerified Customer | Posted 01/24/2020Porcine thymusFixation: -20°C in 100% Methanol for 15 min Primary Ab: 1/100 (+4°C, overnight) Secondary: undiluted, #4001 Agilent (30 min, RT) Developing: AEC Substrate Kit (ab64252 Abcam) incubate for 7 mins at room temperature. Flood with Hematoxylin for 20 secs.
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Application: Western BlotSample Tested: Spleen tissue and Bone marrow cellsSpecies: PigVerified Customer | Posted 01/24/2020WB for porcine spleen lysateSample: 10% porcine spleen lysate in RIPA supplemented with Halt™ Protease Inhibitor Cocktail. Gel: oxidized and denatured protein at loading. No boiling. No freezing-thawing. No pre-diluting of samples. 10% tris/glycine gel. Staining: blocking (5% milk, 1h), primary Ab (R&D #27105-1, 1/150, overnight), secondary Ab (LI-COR #926-32210, 1/10'000, 1h).
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