Rat CCL19/MIP-3 beta Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CCL19/MIP‑3 beta in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 866202
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat CCL19/MIP‑3 beta
    Gly26-Ser108
    Accession # NP_001102131
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.4-2.4 μg/ml in the presence of 25 ng/mL Recombinant Rat CCL19/MIP‑3 beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Rat CCL19/MIP‑3 beta Antibody. Recombinant Rat CCL19/MIP‑3 beta (Catalog # 7800-M3) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL19/MIP‑3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB7800). The ND50 is typically 0.4‑2.4 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta
CCL19 (also known as Mip-3 beta, ELC, CK beta -11, Scya19 and Exodus-3) is a 9 kDa secreted member of the CC family of chemotactic cytokines (1-4). Cells known to express CCL19 are limited in number, and include activated monocytes (5), stromal cells in nodal T zones (6), CD8+ dendritic cells (DC) (6), vascular endothelial cells (7), visceral smooth muscle and mast cells (8), and thymic stromal cells (9).  Rat CCL19 is synthesized as a precursor that is 1108 amino acids (aa) in length. Mature rat CCL19 shares 72% and 89% aa sequence identity with human and mouse CCL19, respectively. CCL19 is known to bind to CCR7 (3, 4), CCLR2/CRAM (L-CCR in rodent) (10, 11) and CCX-CKR (12), with the last two receptors representing scavenger, or chemokine-sink receptors.
  • References:
    1. Sharma, M. (2010) Crit. Rev. Biotechnol. 30:1.
    2. Blanchet, X. et al. (2012) Front. Immunol. 3:175.
    3. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
    4. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
    5. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
    6. Luther, S.A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12694.
    7. Alt, C. et al. (2002) Eur. J. Immunol. 32:2133.
    8. Kaur, D. et al. (2006) Am. J. Respir. Crit. Care Med. 174:1179.
    9. Takamura, K. et al. (2007) J. Immunol. 179:5897.
    10. Leick, M. et al. (2009) Immunology 129:536.
    11. Yoshimura, T. & J.J. Oppenheim (2011) Exp. Cell Res. 317:674.
    12. Comerford, I. et al. (2006) Eur. J. Immunol. 36:1904
  • Entrez Gene IDs:
    6363 (Human); 24047 (Mouse); 362506 (Rat)
  • Alternate Names:
    beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19
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