Rat CCL19/MIP-3 beta Antibody

Catalog #: MAB7800 Datasheet / COA / SDS

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MAB7800
MAB7800-SP

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Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Rat CCL19/MIP‑3 beta Antibody.

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Rat CCL19/MIP-3 beta Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCL19/MIP‑3 beta in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 866202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat CCL19/MIP‑3 beta
Gly26-Ser108
Accession # NP_001102131

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 μg/ml in the presence of 25 ng/mL Recombinant Rat CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Rat CCL19/MIP‑3 beta Antibody. Recombinant Rat CCL19/MIP-3 beta (Catalog # 7800-M3) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB7800). The ND₅₀ is typically 0.4-2.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

CCL19 (also known as Mip-3 beta, ELC, CK beta -11, Scya19 and Exodus-3) is a 9 kDa secreted member of the CC family of chemotactic cytokines (1-4). Cells known to express CCL19 are limited in number, and include activated monocytes (5), stromal cells in nodal T zones (6), CD8⁺ dendritic cells (DC) (6), vascular endothelial cells (7), visceral smooth muscle and mast cells (8), and thymic stromal cells (9). Rat CCL19 is synthesized as a precursor that is 1108 amino acids (aa) in length. Mature rat CCL19 shares 72% and 89% aa sequence identity with human and mouse CCL19, respectively. CCL19 is known to bind to CCR7 (3, 4), CCLR2/CRAM (L-CCR in rodent) (10, 11) and CCX-CKR (12), with the last two receptors representing scavenger, or chemokine-sink receptors.

References

Sharma, M. (2010) Crit. Rev. Biotechnol. 30:1.
Blanchet, X. et al. (2012) Front. Immunol. 3:175.
Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
Luther, S.A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12694.
Alt, C. et al. (2002) Eur. J. Immunol. 32:2133.
Kaur, D. et al. (2006) Am. J. Respir. Crit. Care Med. 174:1179.
Takamura, K. et al. (2007) J. Immunol. 179:5897.
Leick, M. et al. (2009) Immunology 129:536.
Yoshimura, T. & J.J. Oppenheim (2011) Exp. Cell Res. 317:674.
Comerford, I. et al. (2006) Eur. J. Immunol. 36:1904

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names

beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19