Rat CCL20/MIP-3 alpha Antibody

  (1 citations)
(1 Review)
    
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  • Species Reactivity
    Rat
  • Specificity
    Detects rat CCL20/MIP-3 alpha in ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 83225
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant rat CCL20/MIP-3 alpha
    Ala26-Met96
    Accession # P97884.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.20 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM)
    under non-reducing conditions only
    • Rat CCL20/MIP-3 alpha Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Rat CCL20/MIP‑3 alpha Antibody (Catalog # MAB540 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog # BAF540 )
  • ELISA Standard
     
    Recombinant Rat CCL20/MIP-3 alpha Protein (Catalog # 540-RM )
  • Neutralization
    Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 5 µg/mL Recombinant Rat CCL20/MIP‑3 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody. Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP‑3 alpha (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB540). The ND50 is typically 20-40 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% aa sequence homology with mouse and human CCL20, respectively.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  • References:
    1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
    2. Hromas, R. et al. (1997) Blood 89:3315.
    3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
    4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
  • Entrez Gene IDs:
    6364 (Human); 20297 (Mouse); 29538 (Rat)
  • Alternate Names:
    beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells
    Authors: Andy T Y Lau
    Proteomics Clin Appl, 2016;0(0):.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut

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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 148  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Rattus norvegicus.
We have 1 review tested in 1 application: ELISA.

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ELISA Rat CCL20/MIP-3 alpha  Antibody MAB540 Array MAB540
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 ELISA Rattus norvegicus Anonymous 01/11/2017
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ELISA Rat CCL20/MIP-3 alpha  Antibody MAB540
ELISA: Rat CCL20/MIP-3 alpha Antibody [MAB540].

Summary

Application ELISA
Sample Tested Rat lung epithelial cells
Species Rattus norvegicus

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