Rat HSP20/HSPB6 Antibody

Catalog # Availability Size / Price Qty
AF4200
AF4200-SP
Detection of Rat HSP20/HSPB6 by Western Blot.
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Product Details
Citations (3)
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Rat HSP20/HSPB6 Antibody Summary

Species Reactivity
Rat
Specificity
Detects endogenous rat HSP20/HSPB6 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat HSP20/HSPB6
Met1-Lys162
Accession # P97541
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat HSP20/HSPB6 antibody by Western Blot. View Larger

Detection of Rat HSP20/HSPB6 by Western Blot. Western blot shows lysates of rat heart and skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Rat HSP20/HSPB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4200) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP20/HSPB6

Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha -crystallin-related, B6 (HSPB6), is a 162 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Rat HSP20 shares 89 % and 96 % amino acid identity with human and mouse HSP20, respectively.

Long Name
Heat Shock Protein 20
Entrez Gene IDs
126393 (Human); 243912 (Mouse); 192245 (Rat)
Alternate Names
FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; HSP20; HSPB6

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Citations for Rat HSP20/HSPB6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/ alpha B-crystallin after transient middle cerebral artery occlusion in rats
    Authors: Britta Bartelt-Kirbach, Alexander Slowik, Cordian Beyer, Nikola Golenhofen
    Cell Stress and Chaperones
  2. Erratum to: Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons
    Authors: Britta Bartelt-Kirbach, Nikola Golenhofen
    Cell Stress and Chaperones
  3. Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons
    Authors: Britta Bartelt-Kirbach, Nikola Golenhofen
    Cell Stress and Chaperones

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