Rat HSP20/HSPB6 Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects endogenous rat HSP20/HSPB6 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat HSP20/HSPB6
    Met1-Lys162
    Accession # P97541
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat HSP20/HSPB6 by Western Blot. Western blot shows lysates of rat heart and skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Rat HSP20/HSPB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4200) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP20/HSPB6

Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha -crystallin-related, B6 (HSPB6), is a 162 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Rat HSP20 shares 89 % and 96 % amino acid identity with human and mouse HSP20, respectively.

  • Long Name:
    Heat Shock Protein 20
  • Entrez Gene IDs:
    126393 (Human); 243912 (Mouse); 192245 (Rat)
  • Alternate Names:
    FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; HSP20; HSPB6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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