Detection of Rat HSP20/HSPB6 by Western Blot.
Western blot shows lysates of rat heart and skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Rat HSP20/HSPB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4200) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha -crystallin-related, B6 (HSPB6), is a 162 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Rat HSP20 shares 89 % and 96 % amino acid identity with human and mouse HSP20, respectively.
Heat Shock Protein 20
Entrez Gene IDs:
126393 (Human); 243912 (Mouse); 192245 (Rat)
FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; HSP20; HSPB6
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