Reg3B in Rat Pancreas. Reg3B was detected in perfusion fixed frozen sections of rat pancreas using Mouse Anti-Rat Reg3B Monoclonal Antibody (Catalog # MAB1996) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg3B, also named Pancreatitis-Associated Protein (PAP) and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.
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