Rat Serpin A6 Antibody

Catalog # Availability Size / Price Qty
AF3838
AF3838-SP
Detection of Rat Serpin A6 by Western Blot.
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Rat Serpin A6 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Serpin A6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Serpin A1, rmSerpin A5, and recombinant human Serpin A4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Serpin A6
Ser23-Ala396
Accession # NP_001009663
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Serpin A6 antibody by Western Blot. View Larger

Detection of Rat Serpin A6 by Western Blot. Western blot shows lysates of NRK-49F rat fibroblasts and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A6 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A6

Serpin A6 (also corticosteroid-binding globulin/CBG and Transcortin) is a 45‑60 kDa, secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Mature rat Serpin A6 is 374 amino acids (aa) in length (aa 23‑396). It contains an RCL segment that is involved in steroid binding (aa 334‑351). Over aa 23‑396, rat Serpin A6 shares 78% and 60% aa sequence identity with mouse and human Serpin A6, respectively.

Entrez Gene IDs
866 (Human); 12401 (Mouse); 299270 (Rat)
Alternate Names
antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin

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