Rat Synaptotagmin-1 Antibody

(1 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Synaptotagmin‑1 in Western blots. Detects Synaptotagmin-1 from rat, mouse, human, chicken, Xenopus, and fish.
  • Source
    Monoclonal Mouse IgG2A Clone # ASV30
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rat brain synaptic plasma membranes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.

  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Synaptotagmin-1 in Rat Spinal Cord. Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Synaptotagmin-1

The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.

  • Entrez Gene IDs:
    6857 (Human); 20979 (Mouse); 25716 (Rat)
  • Alternate Names:
    DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012;5(0):22.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
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