Rat Synaptotagmin-1 Antibody

Catalog # Availability Size / Price Qty
MAB43641
MAB43641-SP
Synaptotagmin-1 in Rat Spinal Cord.
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Product Details
Citations (1)
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Rat Synaptotagmin-1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Synaptotagmin‑1 in Western blots. Detects Synaptotagmin-1 from rat, mouse, human, chicken, Xenopus, and fish.
Source
Monoclonal Mouse IgG2A Clone # ASV30
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain synaptic plasma membranes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot

Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.

 
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Synaptotagmin-1 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

Synaptotagmin-1 in Rat Spinal Cord. Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptotagmin-1

The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.

Entrez Gene IDs
6857 (Human); 20979 (Mouse); 25716 (Rat)
Alternate Names
DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI

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Citation for Rat Synaptotagmin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012-06-09;5(0):22.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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