Synaptotagmin-1 in Rat Spinal Cord.
Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.
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