Human/Mouse/Rat SOX2 Antibody

(59 citations)
(6 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat SOX2 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 245610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX2
    Gly135-Met317
    Accession # P48431
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    4 µg/mL
    See below
  • CyTOF-reported
    Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  View our protocol for Staining Intracellular Molecules.
Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX2
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  • References:
    1. Graham, V. et al. (2003) Neuron 39:749.
    2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
    3. Kishi, M. et al. (2000) Development 127:791.
    4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
    5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
    6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
  • Long Name:
    Transcription Factor SOX2
  • Entrez Gene IDs:
    6657 (Human)
  • Alternate Names:
    ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

59 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
    Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
    BMC Cancer, 2017;17(1):178.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma
    Authors: R Bayat Mokh, N Baluch, M Ka Hon Tsu, S Kumar, T S Homayoun, K Aitken, B Das, S Baruchel, H Yeger
    BMC Cancer, 2017;17(1):156.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
    Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
    PLoS ONE, 2017;12(2):e0171947.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
    Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
    Stem Cell Reports, 2017;8(1):125-139.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. A Core Regulatory Circuit in Glioblastoma Stem Cells Links MAPK Activation to a Transcriptional Program of Neural Stem Cell Identity
    Authors: G Riddick, S Kotliarova, V Rodriguez, HS Kim, A Linkous, AJ Storaska, S Ahn, J Walling, G Belova, HA Fine
    Sci Rep, 2017;7(0):43605.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Purine synthesis promotes maintenance of brain tumor initiating cells in glioma
    Authors: X Wang, K Yang, Q Xie, Q Wu, SC Mack, Y Shi, LJ Kim, BC Prager, WA Flavahan, X Liu, M Singer, CG Hubert, TE Miller, W Zhou, Z Huang, X Fang, A Regev, ML Suvà, TH Hwang, JW Locasale, S Bao, JN Rich
    Nat. Neurosci, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
    Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
    Stem Cell Res, 2017;21(0):58-70.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Nicotinamide metabolism regulates glioblastoma stem cell maintenance
    Authors: J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich
    JCI Insight, 2017;2(10):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. ODZ1 allows glioblastoma to sustain invasiveness through a Myc-dependent transcriptional upregulation of RhoA
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  11. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A
    Stem Cells Transl Med, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  12. Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
    Authors: Annabelle Congras
    Sci Rep, 2016;6(0):27059.
    Species: Porcine
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  13. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
    PLoS ONE, 2016;11(5):e0155106.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  14. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  15. Enhancing the Three-Dimensional Structure of Adherent Gingival Fibroblasts and Spheroids via a Fibrous Protein-Based Hydrogel Cover
    Cells Tissues Organs (Print), 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  16. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):430-432.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  17. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):441-443.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Fresh
  18. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
    PLoS Biol., 2016;14(11):e1002581.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Sci Rep, 2016;6(0):30742.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  20. SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.
    Authors: Kim, Dohoon, Fiske, Brian P, Birsoy, Kivanc, Freinkman, Elizavet, Kami, Kenjiro, Possemato, Richard, Chudnovsky, Yakov, Pacold, Michael, Chen, Walter W, Cantor, Jason R, Shelton, Laura M, Gui, Dan Y, Kwon, Manjae, Ramkissoon, Shakti H, Ligon, Keith L, Kang, Seong Wo, Snuderl, Matija, Vander Heiden, Matthew, Sabatini, David M
    Nature, 2015;520(7547):363-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  21. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
    Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
    Development, 2015;142(9):1628-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  22. EphA2 promotes infiltrative invasion of glioma stem cells in vivo through cross-talk with Akt and regulates stem cell properties.
    Authors: Miao, H, Gale, N W, Guo, H, Qian, J, Petty, A, Kaspar, J, Murphy, A J, Valenzuela, D M, Yancopoulos, G, Hambardzumyan, D, Lathia, J D, Rich, J N, Lee, J, Wang, B
    Oncogene, 2015;34(5):558-67.
  23. Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape.
    Authors: Sherwood, Richard, Hashimoto, Tatsunor, O'Donnell, Charles, Lewis, Sophia, Barkal, Amira A, van Hoff, John Pet, Karun, Vivek, Jaakkola, Tommi, Gifford, David K
    Nat Biotechnol, 2014;32(2):171-8.
  24. Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.
    Authors: Iacopino F, Angelucci C, Piacentini R, Biamonte F, Mangiola A, Maira G, Grassi C, Sica G
    PLoS ONE, 2014;9(8):e105166.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  25. Fluoxetine induces proliferation and inhibits differentiation of hypothalamic neuroprogenitor cells in vitro.
    Authors: Sousa-Ferreira L, Aveleira C, Botelho M, Alvaro A, Pereira de Almeida L, Cavadas C
    PLoS ONE, 2014;9(3):e88917.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  26. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014;3(1):156-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  27. The subventricular zone is able to respond to a demyelinating lesion after localized radiation.
    Authors: Capilla-Gonzalez V, Guerrero-Cazares H, Bonsu J, Gonzalez-Perez O, Achanta P, Wong J, Garcia-Verdugo J, Quinones-Hinojosa A
    Stem Cells, 2014;32(1):59-69.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  28. Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex.
    Authors: Minamide, Ryohei, Fujiwara, Kazushir, Hasegawa, Koichi, Yoshikawa, Kazuaki
    PLoS ONE, 2014;9(1):e84460.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  29. A single-cell and feeder-free culture system for monkey embryonic stem cells.
    Authors: Ono T, Suzuki Y, Kato Y, Fujita R, Araki T, Yamashita T, Kato H, Torii R, Sato N
    PLoS ONE, 2014;9(2):e88346.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Cells
    Application: ICC
  30. Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
    Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
    Genes Dev, 2014;28(10):1085-100.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  31. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
    Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
    J Immunol, 2014;192(1):523-32.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  32. Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
    Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi
    Sci Rep, 2014;4(0):3701.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IF
  33. Zrf1 is required to establish and maintain neural progenitor identity.
    Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
    Genes Dev, 2014;28(2):182-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  34. Targeted deletion of the ERK5 MAP kinase impairs neuronal differentiation, migration, and survival during adult neurogenesis in the olfactory bulb.
    Authors: Li, Tan, Pan, Yung-Wei, Wang, Wenbin, Abel, Glen, Zou, Junhui, Xu, Lihong, Storm, Daniel R, Xia, Zhengui
    PLoS ONE, 2013;8(4):e61948.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  35. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  36. Fgf10-expressing tanycytes add new neurons to the appetite/energy-balance regulating centers of the postnatal and adult hypothalamus.
    Authors: Haan, Niels, Goodman, Timothy, Najdi-Samiei, Alaleh, Stratford, Christin, Rice, Ritva, El Agha, Elie, Bellusci, Saverio, Hajihosseini, Mohammad
    J Neurosci, 2013;33(14):6170-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  37. Embryonic stem cell-based modeling of tau pathology in human neurons.
    Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
    Am J Pathol, 2013;182(5):1769-79.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  38. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  39. REST regulates oncogenic properties of glioblastoma stem cells.
    Authors: Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H, Fuller GN, Lang FF, Majumder S
    Stem Cells, 2012;30(3):405-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  40. Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel.
    Authors: Hibino N, Villalona G, Pietris N
    FASEB J., 2011;25(8):2731-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  41. Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
    Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
    Stem Cells Dev., 2011;20(6):1079-88.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  42. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  43. Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.
    Authors: Lathia JD, Gallagher J, Myers JT
    PLoS ONE, 2011;6(9):e24807.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  44. Subchronic peripheral neuregulin-1 increases ventral hippocampal neurogenesis and induces antidepressant-like effects.
    Authors: Mahar I, Tan S, Davoli MA, Dominguez-Lopez S, Qiang C, Rachalski A, Turecki G, Mechawar N
    PLoS ONE, 2011;6(10):e26610.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  45. Noninvasive imaging of endogenous neural stem cell mobilization in vivo using positron emission tomography.
    Authors: Rueger MA, Backes H, Walberer M, Neumaier B, Ullrich R, Simard ML, Emig B, Fink GR, Hoehn M, Graf R, Schroeter M
    J. Neurosci., 2010;30(18):6454-60.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  46. Paternal recognition of adult offspring mediated by newly generated CNS neurons.
    Authors: Mak GK, Weiss S,
    Nat. Neurosci., 2010;13(6):753-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  47. Epigenetic changes to human umbilical cord blood cells cultured with three proteins indicate partial reprogramming to a pluripotent state.
    Authors: Wong CJ, Casper RF, Rogers IM
    Exp. Cell Res., 2010;316(6):927-39.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  48. Diagnostic utility of novel stem cell markers SALL4, OCT4, NANOG, SOX2, UTF1, and TCL1 in primary mediastinal germ cell tumors.
    Authors: Liu A, Cheng L, Du J, Peng Y, Allan RW, Wei L, Li J, Cao D
    Am. J. Surg. Pathol., 2010;34(5):697-706.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  49. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2009;28(1):20-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  50. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
    J. Cell. Biochem., 2009;106(5):867-77.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  51. Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation.
    Authors: Lathia JD, Okun E, Tang SC, Griffioen K, Cheng A, Mughal MR, Laryea G, Selvaraj PK, Ffrench-Constant C, Magnus T, Arumugam TV, Mattson MP
    J. Neurosci., 2008;28(51):13978-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  52. Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures.
    Authors: Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD
    Stem Cells, 2008;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  53. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
    Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
    Nat. Cell Biol., 2008;10(7):837-48.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  54. Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria.
    Authors: Gunther HS, Schmidt NO, Phillips HS, Kemming D, Kharbanda S, Soriano R, Modrusan Z, Meissner H, Westphal M, Lamszus K
    Oncogene, 2007;27(20):2897-909.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  55. Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1.
    Authors: Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M
    Biochem. Biophys. Res. Commun., 2007;364(4):737-42.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  56. Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
    Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
    Stem Cells, 2007;25(5):1298-306.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  57. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007;25(6):1511-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  58. Identification of SOX2 as a novel glioma-associated antigen and potential target for T cell-based immunotherapy.
    Authors: Schmitz M, Temme A, Senner V, Ebner R, Schwind S, Stevanovic S, Wehner R, Schackert G, Schackert HK, Fussel M, Bachmann M, Rieber EP, Weigle B
    Br. J. Cancer, 2007;96(8):1293-301.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  59. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
    Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
    Stroke, 2006;37(9):2361-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Average Rating: 4.3 (Based on 6 reviews)

Have you used Human/Mouse/Rat SOX2 Antibody?

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Read what people are saying who have used Human/Mouse/Rat SOX2 Antibody.

We have 6 reviews tested in 2 species: Human, Mouse.
We have 6 reviews tested in 3 applications: Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human/Mouse/Rat SOX2 Antibody MAB2018
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Very Good
 IHC Mouse Anonymous 02/16/2017
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Immunohistochemistry Human/Mouse/Rat SOX2 Antibody MAB2018
Immunohistochemistry: Human/Mouse/Rat SOX2 Antibody [MAB2018]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain (forebrain)
SpeciesMouse

Other Experimental Details

Other Experimental DetailsRed: sox2. Green: ki67. Blue: DAPI. Postnatal day 7 sub-ventricular zone. 2% PFA fixed. Heat-mediated antigen retrieval. Blocked with 10% donkey serum, 0.5% Triton in PBS for 1h. Dilution 1:100.
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX2 Antibody MAB2018
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Excellent
 ICC/IF Human Anonymous 02/03/2017
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Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX2 Antibody MAB2018
Immunocytochemistry/Immunofluorescence: Human/Mouse/Rat SOX2 Antibody [MAB2018]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedNeural progenitor cells
SpeciesHuman
  Very Good
 ICC/IF Human Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedInduced pluripotent stem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsI used this antibody to test Sox2 expression in iPS cells derived from fibroblasts of Sanfilippo syndrome C patients.
Western Blot Human/Mouse SOX2 Antibody MAB2018
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Excellent
 WB Human Anonymous 10/26/2015
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Western Blot Human/Mouse SOX2 Antibody MAB2018
Western Blot: Human/Mouse SOX2 Antibody [MAB2018]

Summary

ApplicationWestern Blot
Sample TestedHuman cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: 5% BSA in TBST
Dilution: 1/1000
Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018
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Very Good
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018
Immunocytochemistry/Immunofluorescence: Human/Mouse SOX2 Antibody [MAB2018]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedhuman NSC
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100
Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018
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Very Good
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018
Immunocytochemistry/Immunofluorescence: Human/Mouse SOX2 Antibody [MAB2018]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedhuman fibroblast and neural stem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100

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