Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Porcine, Feline, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit, Transgenic Mouse

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, COMET, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 245610
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431

Specificity

Detects human, mouse, and rat SOX2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat SOX2 Antibody

Detection of SOX2 in Human Glioblastoma via seqIF™ staining on COMET™​

SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of human Glioblastoma using Mouse Anti-Human SOX2, Monoclonal Antibody (Catalog # MAB2018) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.​​​
E-Cadherin and SOX2 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

E‑Cadherin and SOX2 in BG01V Human Stem Cells.

E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human, Mouse, and Rat SOX2 antibody by Western Blot.

Detection of Human, Mouse, and Rat SOX2 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX2 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX2 in BG01V Human Embryonic Stem Cells.

SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of SOX2 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human SOX2 antibody by Simple WesternTM.

Detection of Human SOX2 by Simple WesternTM.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of SOX2 in glioblastoma.

SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human SOX2 by Western Blot

Detection of Human SOX2 by Western Blot

Western blotting analysis of the different antigens studied in cell lines (NS and AC) and in primary tumors. (a) Stemness antigens and differentiation antigens prevail in NS and AC, respectively. (b) Densitometric quantification of bands in NS and in the corresponding primary tumors. (c) Densitometric quantification of bands in AC and in the corresponding primary tumors. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21869887), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of core and periphery in orthotopic model with five different patient derived GBM spheroid cultures.Tissues were stained with Dapi (blue), Vimentin (green) and Sox-2 (red), Bmi-1 (red) and Glut-3 (red). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both central part and periphery for Sox-2 (G), Bmi-1 (H) and Glut-3 (I). Statistical comparison was performed using student’s t-test. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Soluble factors increase the numbers of Hes3+ cells in the adult rat hippocampus.(a) Treatment with the soluble factors induced a powerful increase in the number of Sox2+ cells in the hilus [Quantifications are from images taken from the tip of the hilus to approximately 1,300 micrometers towards the CA3 region – representative images are shown. Staining for the young neuron marker doublecortin (DCX) helps to visualize the subgranular zone]. (b) The same treatment also induced an increase in the numbers of Hes3+ cells in the hilus. (c) Sox2+ cells inside the hilus co-expressed Hes3; in contrast, Sox2+ cells in the subgranular zone did not co-express Hes3. (d,e) Quantification graphs of the increases in Sox2+ and Hes3+ cell numbers in the hilus (p<0.05). For the graph, the number of cells in the sham operated control animals was set to 100%. [Width of images: a, 650 micrometers; b, 650 micrometers]. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051630), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Soluble factors increase the numbers of Hes3+ cells in the adult rat hippocampus.(a) Treatment with the soluble factors induced a powerful increase in the number of Sox2+ cells in the hilus [Quantifications are from images taken from the tip of the hilus to approximately 1,300 micrometers towards the CA3 region – representative images are shown. Staining for the young neuron marker doublecortin (DCX) helps to visualize the subgranular zone]. (b) The same treatment also induced an increase in the numbers of Hes3+ cells in the hilus. (c) Sox2+ cells inside the hilus co-expressed Hes3; in contrast, Sox2+ cells in the subgranular zone did not co-express Hes3. (d,e) Quantification graphs of the increases in Sox2+ and Hes3+ cell numbers in the hilus (p<0.05). For the graph, the number of cells in the sham operated control animals was set to 100%. [Width of images: a, 650 micrometers; b, 650 micrometers]. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051630), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of core and periphery of patient glioblastomas.Histological sections were stained with Dapi (blue), IDH1 (green) and Sox-2 (red) (AA-BE), Bmi-1 (red) (CA-DE) and Glut-3 (red) (EA to FE). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both core and periphery for Sox-2 (G,J), Bmi-1 (H,K) and Glut-3 (I,L). Statistical comparison was performed using student’s t-test and ANOVA, ** p< 0.01. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Western Blot

Detection of Human SOX2 by Western Blot

CEP-1347 induces the differentiation of cancer stem cells into non-cancer stem cells(A) Glioma stem cells (GS-Y01, GS-Y03, and GS-NCC01), pancreatic cancer stem cells (PANC-1 CSLC), and ovarian cancer stem cells (A2780 CSLC, and TOV21G CSLC) treated without (Control) or with CEP-1347 (300 nM for PANC-1 CSLC, 200 nM for the others) for 6 days were subjected to flow cytometric analysis of the cell-surface expression of CD133. Representative flow cytometric plots together with the percentages of CD133-positive cells are shown. (B) Cells treated as described in (A) were subjected to immunoblot analysis of the indicated proteins. (C) PANC-1 CSLC cells treated without (Control: lane 1) or with CEP-1347 (lane 2) for 6 days and those cultured in the presence of CEP-1347 for 6 days and then in its absence for the indicated days (lanes 3-5) were subjected to immunoblot analysis of the indicated proteins. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22033), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Rat SOX2 by Immunocytochemistry/Immunofluorescence

Fluoxetine inhibits the differentiation of hypothalamic neuroprogenitor cells.Hypothalamic neuroprogenitor cells were differentiated for 18 days, in the presence or absence of fluoxetine (1 µM). Representative confocal photomicrographs for mature neurons marker Neu-N (green) (A). Fluoxetine decreases the percentage of Neu-N positive cells and the incubation with Trk receptors inhibitor K252a (24 h) partially reverses this effect (B). Representative confocal photomicrographs for the progenitor cells marker SOX-2 (green) (C). Fluoxetine increases the percentage of SOX-2 positive cells and the incubation with K252a (24 h) does not change this effect (D). Fluoxetine does not up regulate the levels of neurotrophic factor BDNF but incubation with K252a (24 h) results in a compensatory increase of BDNF mRNA levels (E). DAPI (blue), nuclear staining. Mean ± SEM; n = 4/5; One-Way ANOVA; ns, p>0.05; *, p<0.05; ***, p<0.001 compared to control; NS, p>0.05, #, p<0.05 compared to fluoxetine. Scale bar: 50 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24598761), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Hes3 is a marker of normal and cancer human stem cells.(A–C) Hes3+ cell in the striatum of non-cancerous adult human brain tissue (blood vessels identified by RECA-1 expression), human hemangioblastoma (HBM) biopsy (HBM Hes3+ megakaryocytes shown), human glioblastoma multiforme (GBM) biopsy (Hes3 co-expressed with prominin). (D) Fetal cortical cells sorted for prominin express Sox2 and Hes3. (E) Enrichment for Sox2+ and Hes3+ cells by magnetic sorting using an anti-prominin antibody. (Size bar, 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20195471), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of core and periphery of patient glioblastomas.Histological sections were stained with Dapi (blue), IDH1 (green) and Sox-2 (red) (AA-BE), Bmi-1 (red) (CA-DE) and Glut-3 (red) (EA to FE). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both core and periphery for Sox-2 (G,J), Bmi-1 (H,K) and Glut-3 (I,L). Statistical comparison was performed using student’s t-test and ANOVA, ** p< 0.01. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Immunohistochemistry of a parallel section of Figure 1. (a) Poor GFAP expression. (b) High Nestin expression. (c) SOX2 is highly expressed in nuclei of the external slope of circumscribed necrosis. (d) REST expression, all DAB. (e) CD133-positive area, frozen section. (f) Musashi-1-positive area. Scale bar 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21869887), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with support by a selection of neurotrophic factors. Neural progenitor cells (NPCs) could be cryopreserved at day 21 and plated at day 32 for final experiments, including microelectrode array (MEA) measurements. (b) Three different hPSC lines (one hESC line, 08/023 and two hiPSC lines, 10212.EURCCs and IMR90-4) were characterized for their efficiency in producing neuroectodermal cells in response to 12-day neural induction by dual SMAD inhibition. Cells were stained for pluripotency marker Oct4 at the pluripotent stage and after 12 days of neural induction. The presence of early neuroectodermal markers was evaluated with Sox2, FoxG1 and Pax6 staining. (c) After 21 days of differentiation, the culture contained vimentin- and Pax6-positive NPCs that could be cryopreserved. Additionally, the first Tbr2- and MAP2-positive neurons were detected at this time point. (d) The percentages of Pax6-positive cells were quantified at four time points of differentiation (mean ± s.e.m., n = 5–14, data derived from 1–3 independent differentiations). Statistical analysis was performed with the Mann-Whitney U test to compare differences between day 12 and day 46 within each hPSC line, and significant p-values are presented in the image. The scale bar is 50 µm in all images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31748598), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of core and periphery in orthotopic model with five different patient derived GBM spheroid cultures.Tissues were stained with Dapi (blue), Vimentin (green) and Sox-2 (red), Bmi-1 (red) and Glut-3 (red). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both central part and periphery for Sox-2 (G), Bmi-1 (H) and Glut-3 (I). Statistical comparison was performed using student’s t-test. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Human/Mouse/Rat SOX2 Antibody by Immunocytochemistry/ Immunofluorescence

Detection of Human Human/Mouse/Rat SOX2 Antibody by Immunocytochemistry/ Immunofluorescence

Characterization of TRNDi009-C iPSC line. A) Left: phase contrast imaging of TRNDi009-C colonies grown on Geltrex at passage 6. Right: Representative immunofluorescent images of iPSCs positive for stem cell markers: SOX2, OCT4, NANOG, and SSEA4. Nucleus is labelled with Hoechst 33342 (blue). B) Cytogenetic analysis showing a normal karyotype (46, XX). C) Detection of heterozygous mutation of a p.L302P (c.905 T > C) in exon 2 of the SMPD1 gene. D) Flow cytometry analysis of pluripotency protein markers: NANOG and TRA-1–60. E) RT-PCR verification for the clearance of the Sendai virus from reprogrammed cells. Sendai virus vector transduced fibroblasts were used as a positive control. F) Pathological analysis of teratoma from TRNDi009-C iPSC, showing a normal ectodermal, mesodermal, and endodermal differentiation. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31132580), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Human/Mouse/Rat SOX2 Antibody by Immunocytochemistry/ Immunofluorescence

Detection of Human Human/Mouse/Rat SOX2 Antibody by Immunocytochemistry/ Immunofluorescence

Characterization of TRNDi007-B iPSC line.A) Left: Phase contrast imaging of TRNDi007-B colonies. Right: Representative immunofluorescent images of iPSCs showing expression of stem cell markers: SOX2, OCT4, SSEA-4, and NANOG. Nucleus is stained with Hoechst dye (in blue). B) Flow cytometry analysis of pluripotency protein markers: TRA-1-60, NANOG and SSEA-4. C) Cytogenetic analysis showing a normal karyotype (46, XY). D) Detection of homozygous mutation of c.2560 C > T in exon 18 of the GAA gene. E) RT-PCR verification of the clearance of Sendai virus from the reprogrammed cells. Sendai virus vector transduced fibroblasts was used as positive control. F) Histological analysis of teratomas produced by TRNDi007-B iPSCs. Representative images showing the presence of ectodermal, endodermal and mesodermal derivatives. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31026687), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Human/Mouse/Rat SOX2 Antibody by Western Blot

Detection of Human Human/Mouse/Rat SOX2 Antibody by Western Blot

CEP-1347 induces the differentiation of cancer stem cells into non-cancer stem cells(A) Glioma stem cells (GS-Y01, GS-Y03, and GS-NCC01), pancreatic cancer stem cells (PANC-1 CSLC), and ovarian cancer stem cells (A2780 CSLC, and TOV21G CSLC) treated without (Control) or with CEP-1347 (300 nM for PANC-1 CSLC, 200 nM for the others) for 6 days were subjected to flow cytometric analysis of the cell-surface expression of CD133. Representative flow cytometric plots together with the percentages of CD133-positive cells are shown. (B) Cells treated as described in (A) were subjected to immunoblot analysis of the indicated proteins. (C) PANC-1 CSLC cells treated without (Control: lane 1) or with CEP-1347 (lane 2) for 6 days and those cultured in the presence of CEP-1347 for 6 days and then in its absence for the indicated days (lanes 3-5) were subjected to immunoblot analysis of the indicated proteins. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29212273), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

(A) Differentiation markers for the endoderm (FOXA2, PDX1), mesoderm ( alpha -actin, brachyury), and ectoderm ( beta 3 Tubulin, Otx2) were detected for the MUCG01, MUCG02, and MUCG03 clinical-grade hESC lines by immunocytochemistry. (B) Standard karyotypes without chromosomal aberration were detected for all hESC lines MUCG01: 46,XX; MUCG02: 46,XX; and MUCG03: 46,XY. (C) Pluripotency markers Oct3/4, Sox2, and Nanog were detected for the MUCG01, MUCG02, and MUCG03 clinical-grade hESC lines by immunocytochemistry. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293356), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent Staining of Adult stem cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

Characterization of NS/PCs derived from hiPSCs. b, Representative images (b) of immunocytochemical analysis of hiPSC-NS/PCs (NS/PC-A & NS/PC-B) using antibodies against SOX1, SOX2, & NESTIN. Inset: Hoechst nuclear staining of the same field. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37286713), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOX2 by Western Blot

Detection of SOX2 by Western Blot

Characterization of cell lines.(A) H&E staining of tumor 5653, the arrow indicates the location of the tumor. The right panel shows a higher magnification. (B) Genotyping PCR on genomic wildtype mouse, genomic NPcis mouse (heterozygous for Nf1 and Tp53) DNA and DNA extracted from cell lines 17, 5653 and 5746. The glioma cell lines show complete loss of the wildtype allele for Nf1 and Tp53. (C) Western blot shows the expression of markers consistent with OPC lineage origin of our cell lines. SOX2 is expressed in Neural Progenitor cells (NPC) and Oligodendrocyte precursor cells (OPC); NG2 is expressed in OPCs. Markers (CPNase and MAG) expressed in pre-oligodendrocytes (pre-OL) or mature oligodendrocytes (OL) are not present, highlighting the progenitor status of our lines [22]. (D) The ATRX mutation (E2281*) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with STOP mutation. (E) The homozygous 4bp NF1 deletion (p.LF1247fs*18) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with the homozygous 4bp deletion inducing a frameshift. (F) Western blot for TP53 and CDKN2A, showing that both genes are absent in TM31, but not in glioma line LN319. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36730269), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOX2 by Western Blot

Detection of SOX2 by Western Blot

Characterization of cell lines.(A) H&E staining of tumor 5653, the arrow indicates the location of the tumor. The right panel shows a higher magnification. (B) Genotyping PCR on genomic wildtype mouse, genomic NPcis mouse (heterozygous for Nf1 and Tp53) DNA and DNA extracted from cell lines 17, 5653 and 5746. The glioma cell lines show complete loss of the wildtype allele for Nf1 and Tp53. (C) Western blot shows the expression of markers consistent with OPC lineage origin of our cell lines. SOX2 is expressed in Neural Progenitor cells (NPC) and Oligodendrocyte precursor cells (OPC); NG2 is expressed in OPCs. Markers (CPNase and MAG) expressed in pre-oligodendrocytes (pre-OL) or mature oligodendrocytes (OL) are not present, highlighting the progenitor status of our lines [22]. (D) The ATRX mutation (E2281*) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with STOP mutation. (E) The homozygous 4bp NF1 deletion (p.LF1247fs*18) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with the homozygous 4bp deletion inducing a frameshift. (F) Western blot for TP53 and CDKN2A, showing that both genes are absent in TM31, but not in glioma line LN319. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36730269), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

Detection of Human SOX2 by Immunocytochemistry/ Immunofluorescence

Characterization of NS/PCs derived from hiPSCs. b, Representative images (b) of immunocytochemical analysis of hiPSC-NS/PCs (NS/PC-A & NS/PC-B) using antibodies against SOX1, SOX2, & NESTIN. Inset: Hoechst nuclear staining of the same field. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37286713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat SOX2 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-reported

Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed NTera‑2 human embryonic carcinoma cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of glioblastoma

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: NTera‑2 human embryonic carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Multiplex Immunofluorescence

5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Glioblastoma

Simple Western

4 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells

Reviewed Applications

Read 17 reviews rated 4.5 using MAB2018 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

References

  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Entrez Gene IDs

6657 (Human)

Gene Symbol

SOX2

UniProt

P48431

Additional SOX2 Products

Product Documents for Human/Mouse/Rat SOX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat SOX2 Antibody

For research use only

Citations for Human/Mouse/Rat SOX2 Antibody

Customer Reviews for Human/Mouse/Rat SOX2 Antibody (17)

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  • Human/Mouse/Rat SOX2 Antibody
    Name: Edma Loku
    Application: Immunofluorescence staining
    Sample Tested: Embryonic lung
    Species: Mouse
    Verified Customer | Posted 10/30/2025
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 11/28/2024
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: BG01V human embryonic stem cells
    Species: Human
    Verified Customer | Posted 11/21/2024
    Concentration1:200 Human sample
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT-29 human colon adenocarcinoma cell line and HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 10/24/2023
    SOX2 expression was examined by WB on two colorectal cancer cell lines. A clear band at 35KD was observed.
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: glioblastoma cells
    Species: Human
    Verified Customer | Posted 08/06/2021
    On the photo SOX2 is red, nuclear DAPI is blue.
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Shuba Krishnan
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 10/18/2018
    Cells: human induced pluripotent stem cells Primary antibody: anti-human SOX2 (1:200), O/N incubation at 4 degrees Secondary antibody: anti-mouse AF488
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain (forebrain)
    Species: Mouse
    Verified Customer | Posted 02/16/2017
    Red: sox2. Green: ki67. Blue: DAPI. Postnatal day 7 sub-ventricular zone. 2% PFA fixed. Heat-mediated antigen retrieval. Blocked with 10% donkey serum, 0.5% Triton in PBS for 1h. Dilution 1:100.
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 02/03/2017
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    I used this antibody to test Sox2 expression in iPS cells derived from fibroblasts of Sanfilippo syndrome C patients.
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human fibroblast and neural stem cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: 1% BSA + 0.3% Triton X-100 in PBS<br />Dilution: 1/100
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 5% BSA in TBST<br />Dilution: 1/1000
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human NSC
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: 1% BSA + 0.3% Triton X-100 in PBS<br />Dilution: 1/100
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22791629
    Species: Rat
    Verified Customer | Posted 02/16/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 19824823
    Species: Human
    Verified Customer | Posted 02/16/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 19825428
    Species: Mouse
    Verified Customer | Posted 02/16/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23658023
    Species: Human
    Verified Customer | Posted 02/16/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22573702
    Species: Other
    Verified Customer | Posted 02/16/2015

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FAQs for Human/Mouse/Rat SOX2 Antibody

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  • Q: What is the light chain of Human/Mouse/Rat SOX2 Antibody, Catalog #s MAB2018, MAB2018R and IC2018?

    A: Catalog #s MAB2018, MAB2018R and IC2018 all have a kappa light chain.

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