Home / SOX2 / Human/Mouse/Rat SOX2 Antibody

Human/Mouse/Rat SOX2 Antibody

MAB2018 97 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2018
MAB2018-SP
E‑Cadherin and SOX2 in BG01V Human Stem Cells.
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Citations (97)
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Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SOX2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 245610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
4 µg/mL
See below
CyTOF-reported
Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry E‑Cadherin and SOX2 in BG01V Human Stem Cells. View Larger

E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human, Mouse, and Rat SOX2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SOX2 in BG01V Human Embryonic Stem Cells. View Larger

SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. View Larger

Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human SOX2 by Simple WesternTM. View Larger

Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

References
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Entrez Gene IDs
6657 (Human)
Alternate Names
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2

Product Datasheets

Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat SOX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

97 Citations: Showing 1 - 10
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  1. Single-cell transcriptomic analysis of mouse neocortical development
    Authors: L Loo, JM Simon, L Xing, ES McCoy, JK Niehaus, J Guo, ES Anton, MJ Zylka
    Nat Commun, 2019;10(1):134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death
    Authors: MA Ajmone-Cat, A Onori, C Toselli, E Stronati, M Morlando, I Bozzoni, E Monni, Z Kokaia, G Lupo, L Minghetti, S Biagioni, E Cacci
    Sci Rep, 2019;9(1):4572.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer
    Authors: S Melissarid, E Wiechec, M Magan, MV Jain, MK Chung, L Farnebo, K Roberg
    Cancer Cell Int., 2019;19(0):16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Generation of two induced pluripotent stem cell lines from skin fibroblasts of sisters carrying a c.1094C>A variation in the SCN10A gene potentially associated with small fiber neuropathy
    Authors: T Klein, K Klug, L Henkel, CK Kwok, F Edenhofer, E Klopocki, I Kurth, N Üçeyler
    Stem Cell Res, 2019;35(0):101396.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi007-A from a Long QT Syndrome type 1 patient carrier of two common variants in the NOS1AP gene
    Authors: M Mura, F Pisano, M Stefanello, M Ginevrino, M Boni, F Calabrò, L Crotti, EM Valente, PJ Schwartz, PA Brink, M Gnecchi
    Stem Cell Res, 2019;36(0):101416.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene
    Authors: W Huang, M Xu, R Li, A Baskfield, J Kouznetsov, J Beers, J Zou, C Liu, W Zheng
    Stem Cell Res, 2019;37(0):101427.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Generation of an induced pluripotent stem cell line (TRNDi005-A) from a Mucopolysaccharidosis Type IVA (MPS IVA) patient carrying compound heterozygous p.R61W and p.WT405del mutations in the GALNS gene
    Authors: R Li, A Baskfield, J Beers, J Zou, C Liu, CJ Alméciga-D, W Zheng
    Stem Cell Res, 2019;36(0):101408.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
    Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
    Sci Rep, 2019;9(1):4902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids
    Authors: H Kim, HJ Park, H Choi, Y Chang, H Park, J Shin, J Kim, CJ Lengner, YK Lee, J Kim
    Stem Cell Reports, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma
    Authors: M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson
    Gynecol. Oncol., 2019;0(0):.
    Species: Human
    Sample Types: Tumor Tissue Fluid
    Applications: ICC
  11. Generation of an induced pluripotent stem cell line (TRNDi002-B) from a patient carrying compound heterozygous p.Q208X and p.G310G mutations in the NGLY1 gene
    Authors: R Li, M Pradhan, M Xu, A Baskfield, A Farkhondeh, YS Cheng, J Beers, J Zou, C Liu, M Might, S Rodems, W Zheng
    Stem Cell Res, 2018;34(0):101362.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  12. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation
    Authors: M Naumann, A Pal, A Goswami, X Lojewski, J Japtok, A Vehlow, M Naujock, R Günther, M Jin, N Stanslowsk, P Reinhardt, J Sternecker, M Frickenhau, F Pan-Montoj, E Storkebaum, I Poser, A Freischmid, JH Weishaupt, K Holzmann, D Troost, AC Ludolph, TM Boeckers, S Liebau, S Petri, N Cordes, AA Hyman, F Wegner, SW Grill, J Weis, A Storch, A Hermann
    Nat Commun, 2018;9(1):335.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Generation of an induced pluripotent stem cell line (TRNDi003-A) from a Noonan syndrome with multiple lentigines (NSML) patient carrying a p.Q510P mutation in the PTPN11 gene
    Authors: R Li, A Baskfield, Y Lin, J Beers, J Zou, C Liu, F Jaffré, AE Roberts, EA Ottinger, MI Kontaridis, W Zheng
    Stem Cell Res, 2018;34(0):101374.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  14. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1
    Authors: M Mura, M Ginevrino, R Zappatore, F Pisano, M Boni, S Castellett, L Crotti, EM Valente, PJ Schwartz, M Gnecchi
    Stem Cell Res, 2018;29(0):170-173.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
    Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
    Nat. Cell Biol., 2018;20(5):553-564.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018;22(4):543-558.e12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation
    Authors: CP Cui, Y Zhang, C Wang, F Yuan, H Li, Y Yao, Y Chen, C Li, W Wei, CH Liu, F He, Y Liu, L Zhang
    Nat Commun, 2018;9(1):4648.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  18. Generation of two induced pluripotent stem cells lines from a Mucopolysaccharydosis IIIB (MPSIIIB) patient
    Authors: S Vallejo-Di, A Fleischer, JM Martín-Fer, A Sánchez-Gi, D Bachiller
    Stem Cell Res, 2018;33(0):180-184.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  19. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease
    Authors: JY Lee, SH Han, MH Park, B Baek, IS Song, MK Choi, Y Takuwa, H Ryu, SH Kim, X He, EH Schuchman, JS Bae, HK Jin
    Nat Commun, 2018;9(1):1479.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas
    Authors: C Aaberg-Jes, MD Sørensen, ALSA Matos, JM Moreira, N Brünner, A Knudsen, BW Kristensen
    BMC Cancer, 2018;18(1):270.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation
    Authors: S Kwak, TW Kim, BH Kang, JH Kim, JS Lee, HT Lee, IY Hwang, J Shin, JH Lee, EJ Cho, HD Youn
    Nucleic Acids Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury
    Authors: A Alastrue-A, FJ Rodriguez-, EL Mocholi, F De Giorgio, S Erceg, V Moreno-Man
    Int J Mol Sci, 2018;19(1):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells
    Authors: M Jeong, M O'Reilly, NK Kirkwood, J Al-Aama, M Lako, CJ Kros, L Armstrong
    Cell Death Dis, 2018;9(9):922.
    Species: Human
    Sample Types: Tissue Section
    Applications: IHC
  24. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice
    Authors: O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour
    Stem Cell Reports, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Generation of the human induced pluripotent stem cell line (UKWNLi001-A) from skin fibroblasts of a woman with Fabry disease carrying the X-chromosomal heterozygous c.708?G?>?C (W236C) missense mutation in exon 5 of the alpha-galactosidase-A gene
    Authors: T Klein, K Günther, CK Kwok, F Edenhofer, N Üçeyler
    Stem Cell Res, 2018;31(0):222-226.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018;15(1):226.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  28. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
    Authors: R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura
    Sci Rep, 2018;8(1):12187.
    Species: Rhesus Macaque
    Sample Types: Whole Cells
    Applications: ICC
  29. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi002-A from a patient affected by the Jervell and Lange-Nielsen syndrome and carrier of two compound heterozygous mutations on the KCNQ1 gene
    Authors: M Mura, YK Lee, M Ginevrino, R Zappatore, F Pisano, M Boni, F Dagradi, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi
    Stem Cell Res, 2018;29(0):157-161.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Repositioning CEP-1347, a chemical agent originally developed for the treatment of Parkinson's disease, as an anti-cancer stem cell drug
    Authors: M Okada, H Takeda, H Sakaki, K Kuramoto, S Suzuki, T Sanomachi, K Togashi, S Seino, C Kitanaka
    Oncotarget, 2017;8(55):94872-94882.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding
    Authors: S Falk, S Bugeon, J Ninkovic, GA Pilz, MP Postiglion, H Cremer, JA Knoblich, M Götz
    Neuron, 2017;93(4):777-791.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
    Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li
    Stem Cell Res, 2017;22(0):29-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix
    Authors: G Kaufman, D Skrtic
    Tissue Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  34. Purine synthesis promotes maintenance of brain tumor initiating cells in glioma
    Authors: X Wang, K Yang, Q Xie, Q Wu, SC Mack, Y Shi, LJ Kim, BC Prager, WA Flavahan, X Liu, M Singer, CG Hubert, TE Miller, W Zhou, Z Huang, X Fang, A Regev, ML Suvà, TH Hwang, JW Locasale, S Bao, JN Rich
    Nat. Neurosci, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
    Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
    Stem Cell Reports, 2017;8(1):125-139.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Nicotinamide metabolism regulates glioblastoma stem cell maintenance
    Authors: J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich
    JCI Insight, 2017;2(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma
    Authors: R Bayat Mokh, N Baluch, M Ka Hon Tsu, S Kumar, T S Homayoun, K Aitken, B Das, S Baruchel, H Yeger
    BMC Cancer, 2017;17(1):156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. A Core Regulatory Circuit in Glioblastoma Stem Cells Links MAPK Activation to a Transcriptional Program of Neural Stem Cell Identity
    Authors: G Riddick, S Kotliarova, V Rodriguez, HS Kim, A Linkous, AJ Storaska, S Ahn, J Walling, G Belova, HA Fine
    Sci Rep, 2017;7(0):43605.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Laminin ?5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro
    Authors: A Hyysalo, M Ristola, ME Mäkinen, S Häyrynen, M Nykter, S Narkilahti
    Stem Cell Res, 2017;24(0):118-127.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
    Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
    Stem Cell Res, 2017;21(0):58-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1
    Authors: K Hirano, M Namihira
    FEBS Open Bio, 2017;7(12):1932-1942.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
    Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
    PLoS ONE, 2017;12(2):e0171947.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
    Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
    Circ. Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  45. Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression
    Authors: AG Alvarado, PS Thiagaraja, EE Mulkearns-, DJ Silver, JS Hale, TJ Alban, SM Turaga, A Jarrar, O Reizes, MS Longworth, MA Vogelbaum, JD Lathia
    Cell Stem Cell, 2017;20(4):450-461.e4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  46. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
    Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
    Cell Death Discov, 2017;3(0):16105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  47. Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
    Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
    BMC Cancer, 2017;17(1):178.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  48. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A
    Stem Cells Transl Med, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  49. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):430-432.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  50. ODZ1 allows glioblastoma to sustain invasiveness through a Myc-dependent transcriptional upregulation of RhoA
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  51. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):441-443.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Fresh
  52. Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
    Authors: Annabelle Congras
    Sci Rep, 2016;6(0):27059.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: IHC - PFA fixed
  53. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
    PLoS ONE, 2016;11(5):e0155106.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  54. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  55. Enhancing the Three-Dimensional Structure of Adherent Gingival Fibroblasts and Spheroids via a Fibrous Protein-Based Hydrogel Cover
    Cells Tissues Organs (Print), 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  56. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Sci Rep, 2016;6(0):30742.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Fresh
  57. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
    PLoS Biol., 2016;14(11):e1002581.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.
    Authors: Kim, Dohoon, Fiske, Brian P, Birsoy, Kivanc, Freinkman, Elizavet, Kami, Kenjiro, Possemato, Richard, Chudnovsky, Yakov, Pacold, Michael, Chen, Walter W, Cantor, Jason R, Shelton, Laura M, Gui, Dan Y, Kwon, Manjae, Ramkissoon, Shakti H, Ligon, Keith L, Kang, Seong Wo, Snuderl, Matija, Vander Heiden, Matthew, Sabatini, David M
    Nature, 2015;520(7547):363-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
    Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
    Development, 2015;142(9):1628-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  60. EphA2 promotes infiltrative invasion of glioma stem cells in vivo through cross-talk with Akt and regulates stem cell properties.
    Authors: Miao, H, Gale, N W, Guo, H, Qian, J, Petty, A, Kaspar, J, Murphy, A J, Valenzuela, D M, Yancopoulos, G, Hambardzumyan, D, Lathia, J D, Rich, J N, Lee, J, Wang, B
    Oncogene, 2015;34(5):558-67.
  61. Zrf1 is required to establish and maintain neural progenitor identity.
    Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
    Genes Dev, 2014;28(2):182-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  62. Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape.
    Authors: Sherwood, Richard, Hashimoto, Tatsunor, O'Donnell, Charles, Lewis, Sophia, Barkal, Amira A, van Hoff, John Pet, Karun, Vivek, Jaakkola, Tommi, Gifford, David K
    Nat Biotechnol, 2014;32(2):171-8.
  63. Fluoxetine induces proliferation and inhibits differentiation of hypothalamic neuroprogenitor cells in vitro.
    Authors: Sousa-Ferreira L, Aveleira C, Botelho M, Alvaro A, Pereira de Almeida L, Cavadas C
    PLoS ONE, 2014;9(3):e88917.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  64. Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
    Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi
    Sci Rep, 2014;4(0):3701.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunofluorescence
  65. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014;3(1):156-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  66. A single-cell and feeder-free culture system for monkey embryonic stem cells.
    Authors: Ono T, Suzuki Y, Kato Y, Fujita R, Araki T, Yamashita T, Kato H, Torii R, Sato N
    PLoS ONE, 2014;9(2):e88346.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  67. Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
    Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
    Genes Dev, 2014;28(10):1085-100.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunofluorescence
  68. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
    Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
    J Immunol, 2014;192(1):523-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunofluorescence
  69. Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.
    Authors: Iacopino F, Angelucci C, Piacentini R, Biamonte F, Mangiola A, Maira G, Grassi C, Sica G
    PLoS ONE, 2014;9(8):e105166.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  70. Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex.
    Authors: Minamide, Ryohei, Fujiwara, Kazushir, Hasegawa, Koichi, Yoshikawa, Kazuaki
    PLoS ONE, 2014;9(1):e84460.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  71. The subventricular zone is able to respond to a demyelinating lesion after localized radiation.
    Authors: Capilla-Gonzalez V, Guerrero-Cazares H, Bonsu J, Gonzalez-Perez O, Achanta P, Wong J, Garcia-Verdugo J, Quinones-Hinojosa A
    Stem Cells, 2014;32(1):59-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  72. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  73. Embryonic stem cell-based modeling of tau pathology in human neurons.
    Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
    Am J Pathol, 2013;182(5):1769-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fluorescence
  74. Targeted deletion of the ERK5 MAP kinase impairs neuronal differentiation, migration, and survival during adult neurogenesis in the olfactory bulb.
    Authors: Li, Tan, Pan, Yung-Wei, Wang, Wenbin, Abel, Glen, Zou, Junhui, Xu, Lihong, Storm, Daniel R, Xia, Zhengui
    PLoS ONE, 2013;8(4):e61948.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  75. Fgf10-expressing tanycytes add new neurons to the appetite/energy-balance regulating centers of the postnatal and adult hypothalamus.
    Authors: Haan, Niels, Goodman, Timothy, Najdi-Samiei, Alaleh, Stratford, Christin, Rice, Ritva, El Agha, Elie, Bellusci, Saverio, Hajihosseini, Mohammad
    J Neurosci, 2013;33(14):6170-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  76. REST regulates oncogenic properties of glioblastoma stem cells.
    Authors: Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H, Fuller GN, Lang FF, Majumder S
    Stem Cells, 2012;30(3):405-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  77. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel.
    Authors: Hibino N, Villalona G, Pietris N
    FASEB J., 2011;25(8):2731-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  79. Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
    Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
    Stem Cells Dev., 2011;20(6):1079-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  80. Subchronic peripheral neuregulin-1 increases ventral hippocampal neurogenesis and induces antidepressant-like effects.
    Authors: Mahar I, Tan S, Davoli MA, Dominguez-Lopez S, Qiang C, Rachalski A, Turecki G, Mechawar N
    PLoS ONE, 2011;6(10):e26610.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  81. Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.
    Authors: Lathia JD, Gallagher J, Myers JT
    PLoS ONE, 2011;6(9):e24807.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  82. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2011;19(3):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  83. Epigenetic changes to human umbilical cord blood cells cultured with three proteins indicate partial reprogramming to a pluripotent state.
    Authors: Wong CJ, Casper RF, Rogers IM
    Exp. Cell Res., 2010;316(6):927-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Diagnostic utility of novel stem cell markers SALL4, OCT4, NANOG, SOX2, UTF1, and TCL1 in primary mediastinal germ cell tumors.
    Authors: Liu A, Cheng L, Du J, Peng Y, Allan RW, Wei L, Li J, Cao D
    Am. J. Surg. Pathol., 2010;34(5):697-706.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  85. Noninvasive imaging of endogenous neural stem cell mobilization in vivo using positron emission tomography.
    Authors: Rueger MA, Backes H, Walberer M, Neumaier B, Ullrich R, Simard ML, Emig B, Fink GR, Hoehn M, Graf R, Schroeter M
    J. Neurosci., 2010;30(18):6454-60.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  86. Paternal recognition of adult offspring mediated by newly generated CNS neurons.
    Authors: Mak GK, Weiss S,
    Nat. Neurosci., 2010;13(6):753-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  87. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2009;28(1):20-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  88. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
    J. Cell. Biochem., 2009;106(5):867-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  89. Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures.
    Authors: Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD
    Stem Cells, 2008;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  90. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
    Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
    Nat. Cell Biol., 2008;10(7):837-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  91. Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation.
    Authors: Lathia JD, Okun E, Tang SC, Griffioen K, Cheng A, Mughal MR, Laryea G, Selvaraj PK, Ffrench-Constant C, Magnus T, Arumugam TV, Mattson MP
    J. Neurosci., 2008;28(51):13978-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  92. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007;25(6):1511-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria.
    Authors: Gunther HS, Schmidt NO, Phillips HS, Kemming D, Kharbanda S, Soriano R, Modrusan Z, Meissner H, Westphal M, Lamszus K
    Oncogene, 2007;27(20):2897-909.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  94. Identification of SOX2 as a novel glioma-associated antigen and potential target for T cell-based immunotherapy.
    Authors: Schmitz M, Temme A, Senner V, Ebner R, Schwind S, Stevanovic S, Wehner R, Schackert G, Schackert HK, Fussel M, Bachmann M, Rieber EP, Weigle B
    Br. J. Cancer, 2007;96(8):1293-301.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  95. Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
    Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
    Stem Cells, 2007;25(5):1298-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  96. Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1.
    Authors: Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M
    Biochem. Biophys. Res. Commun., 2007;364(4):737-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  97. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
    Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
    Stroke, 2006;37(9):2361-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse/Rat SOX2 Antibody

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Human/Mouse/Rat SOX2 Antibody
By Anonymous on 10/18/2018
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

Cells: human induced pluripotent stem cells
Primary antibody: anti-human SOX2 (1:200), O/N incubation at 4 degrees
Secondary antibody: anti-mouse AF488

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX2 Antibody MAB2018
Human/Mouse/Rat SOX2 Antibody
By Anonymous on 02/16/2017
Application: IHC Sample Tested: Brain (forebrain) Species: Mouse

Red: sox2. Green: ki67. Blue: DAPI. Postnatal day 7 sub-ventricular zone. 2% PFA fixed. Heat-mediated antigen retrieval. Blocked with 10% donkey serum, 0.5% Triton in PBS for 1h. Dilution 1:100.

Immunohistochemistry Human/Mouse/Rat SOX2 Antibody MAB2018
Human/Mouse/Rat SOX2 Antibody
By Anonymous on 02/03/2017
Application: ICC/IF Sample Tested: Neural progenitor cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX2 Antibody MAB2018
Human/Mouse SOX2 Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Induced pluripotent stem cells Species: Human

I used this antibody to test Sox2 expression in iPS cells derived from fibroblasts of Sanfilippo syndrome C patients.

Human/Mouse SOX2 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Human cells Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: 5% BSA in TBST
Dilution: 1/1000

Western Blot Human/Mouse SOX2 Antibody MAB2018
Human/Mouse SOX2 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: human NSC Species: Human

Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100

Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018
Human/Mouse SOX2 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: human fibroblast and neural stem cells Species: Human

Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100

Immunocytochemistry/Immunofluorescence Human/Mouse SOX2 Antibody MAB2018