Human/Mouse/Rat SOX2 Antibody Summary
Gly135-Met317
Accession # P48431
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

E‑Cadherin and SOX2 in BG01V Human Stem Cells. E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX2
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
- Graham, V. et al. (2003) Neuron 39:749.
- Avilion, A.A. et al. (2003) Genes Dev. 17:126.
- Kishi, M. et al. (2000) Development 127:791.
- Yuan, H. et al. (1995) Genes Dev. 9:2635.
- Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
- Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Product Datasheets
Citations for Human/Mouse/Rat SOX2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
Cell Rep, 2020;31(5):107599.
Species: Human
Sample Types:
Applications: IF -
Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death
Authors: MA Ajmone-Cat, A Onori, C Toselli, E Stronati, M Morlando, I Bozzoni, E Monni, Z Kokaia, G Lupo, L Minghetti, S Biagioni, E Cacci
Sci Rep, 2019;9(1):4572.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene
Authors: J Hong, M Xu, R Li, YS Cheng, J Kouznetsov, J Beers, C Liu, J Zou, W Zheng
Stem Cell Res, 2019;37(0):101451.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Yin Yang 1 sustains biosynthetic demands during brain development in a stage-specific manner
Authors: L Zurkirchen, S Varum, S Giger, A Klug, J Häusel, R Bossart, M Zemke, C Cantù, ZK Atak, N Zamboni, K Basler, L Sommer
Nat Commun, 2019;10(1):2192.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
User-defined morphogen patterning for directing human cell fate stratification
Authors: MC Regier, JJ Tokar, JW Warrick, L Pabon, E Berthier, DJ Beebe, KR Stevens
Sci Rep, 2019;9(1):6433.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A human induced pluripotent stem cell line (TRNDi007-B) from an infantile onset Pompe patient carrying p.R854X mutation in the GAA gene
Authors: YS Cheng, R Li, A Baskfield, J Beers, J Zou, C Liu, W Zheng
Stem Cell Res, 2019;37(0):101435.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Single-cell transcriptomic analysis of mouse neocortical development
Authors: L Loo, JM Simon, L Xing, ES McCoy, JK Niehaus, J Guo, ES Anton, MJ Zylka
Nat Commun, 2019;10(1):134.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma
Authors: M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson
Gynecol. Oncol., 2019;0(0):.
Species: Human
Sample Types: Tumor Tissue Fluid
Applications: ICC -
A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
Sci Rep, 2019;9(1):4902.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p.L43_A44delLA in the SMPD1 gene
Authors: A Baskfield, R Li, J Beers, J Zou, C Liu, W Zheng
Stem Cell Res, 2019;37(0):101436.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an induced pluripotent stem cell line (TRNDi005-A) from a Mucopolysaccharidosis Type IVA (MPS IVA) patient carrying compound heterozygous p.R61W and p.WT405del mutations in the GALNS gene
Authors: R Li, A Baskfield, J Beers, J Zou, C Liu, CJ Alméciga-D, W Zheng
Stem Cell Res, 2019;36(0):101408.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene
Authors: W Huang, M Xu, R Li, A Baskfield, J Kouznetsov, J Beers, J Zou, C Liu, W Zheng
Stem Cell Res, 2019;37(0):101427.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi007-A from a Long QT Syndrome type 1 patient carrier of two common variants in the NOS1AP gene
Authors: M Mura, F Pisano, M Stefanello, M Ginevrino, M Boni, F Calabrò, L Crotti, EM Valente, PJ Schwartz, PA Brink, M Gnecchi
Stem Cell Res, 2019;36(0):101416.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids
Authors: H Kim, HJ Park, H Choi, Y Chang, H Park, J Shin, J Kim, CJ Lengner, YK Lee, J Kim
Stem Cell Reports, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of two induced pluripotent stem cell lines from skin fibroblasts of sisters carrying a c.1094C>A variation in the SCN10A gene potentially associated with small fiber neuropathy
Authors: T Klein, K Klug, L Henkel, CK Kwok, F Edenhofer, E Klopocki, I Kurth, N Üçeyler
Stem Cell Res, 2019;35(0):101396.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer
Authors: S Melissarid, E Wiechec, M Magan, MV Jain, MK Chung, L Farnebo, K Roberg
Cancer Cell Int., 2019;19(0):16.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation
Authors: M Mura, YK Lee, F Pisano, M Ginevrino, M Boni, F Calabrò, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi
Stem Cell Res, 2019;37(0):101437.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi004-A from a carrier of the KCNQ1-R594Q mutation
Authors: M Mura, YK Lee, F Pisano, M Ginevrino, M Boni, F Calabrò, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi
Stem Cell Res, 2019;37(0):101431.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The use of simultaneous reprogramming and gene correction to generate an osteogenesis imperfecta patient COL1A1 c. 3936 G>T iPSC line and an isogenic control iPSC line
Authors: S Howden, H Hosseini F, A Motazedian, AG Elefanty, EG Stanley, SR Lamandé, JF Bateman
Stem Cell Res, 2019;38(0):101453.
Species: Human
Sample Types: Whole Cells
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An induced pluripotent stem cell line (TRNDi010-C) from a patient carrying a homozygous p.R401X mutation in the NGLY1 gene
Authors: S Yang, YS Cheng, R Li, M Pradhan, J Hong, J Beers, J Zou, C Liu, M Might, S Rodems, W Zheng
Stem Cell Res, 2019;39(0):101496.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
Authors: T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos, A Vinogradov, SJ Eglen, L Ylä-Outine, S Narkilahti
Sci Rep, 2019;9(1):17125.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi006-A from a patient affected by an autosomal recessive form of long QT syndrome type 1
Authors: M Mura, F Bastaroli, M Corli, M Ginevrino, F Calabrò, M Boni, L Crotti, EM Valente, PJ Schwartz, M Gnecchi
Stem Cell Res, 2019;42(0):101658.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
An induced pluripotent stem cell line (TRNDi009-C) from a Niemann-Pick disease type A patient carrying a heterozygous p.L302P (c.905 T>C) mutation in the SMPD1 gene
Authors: A Baskfield, R Li, J Beers, J Zou, C Liu, W Zheng
Stem Cell Res, 2019;38(0):101461.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs
Authors: E Grall, V Gourain, A Naïr, E Martin, MC Birling, JN Freund, I Duluc
Cell Death Dis, 2019;10(11):812.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Pathogenic mutations in neurofibromin identifies a leucine-rich domain regulating glioma cell invasiveness
Authors: SFB Fadhlullah, NBA Halim, JYT Yeo, RLY Ho, P Um, BT Ang, C Tang, WH Ng, DM Virshup, IAW Ho
Oncogene, 2019;38(27):5367-5380.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
ER stress and UPR activation in glioblastoma: identification of a noncanonical PERK mechanism regulating GBM stem cells through SOX2 modulation
Authors: NM Peñaranda-, C Meijer, Y Liang, BM Dijkstra, R Aguirre-Ga, WFA den Dunnen, FAE Kruyt
Cell Death Dis, 2019;10(10):690.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells
Authors: A Stubb, C Guzmán, E Närvä, J Aaron, TL Chew, M Saari, M Miihkinen, G Jacquemet, J Ivaska
Nat Commun, 2019;10(1):4756.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons
Authors: C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio
Sci Rep, 2019;9(1):6811.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
Cell Stem Cell, 2018;22(4):543-558.e12.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Differential response in novel stem cell niches of the brain following cervical spinal cord injury and traumatic brain injury
Authors: A Falnikar, J Stratton, R Lin, CE Andrews, A Tyburski, VA Trovillion, C Gottschalk, B Ghosh, L Iacovitti, MB Elliott, AC Lepore
J. Neurotrauma, 2018;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas
Authors: C Aaberg-Jes, MD Sørensen, ALSA Matos, JM Moreira, N Brünner, A Knudsen, BW Kristensen
BMC Cancer, 2018;18(1):270.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease
Authors: JY Lee, SH Han, MH Park, B Baek, IS Song, MK Choi, Y Takuwa, H Ryu, SH Kim, X He, EH Schuchman, JS Bae, HK Jin
Nat Commun, 2018;9(1):1479.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury
Authors: A Alastrue-A, FJ Rodriguez-, EL Mocholi, F De Giorgio, S Erceg, V Moreno-Man
Int J Mol Sci, 2018;19(1):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
Authors: X Gao, JJ Yourick, RL Sprando
Stem Cell Res, 2018;31(0):193-196.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
Authors: R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura
Sci Rep, 2018;8(1):12187.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1
Authors: M Mura, M Ginevrino, R Zappatore, F Pisano, M Boni, S Castellett, L Crotti, EM Valente, PJ Schwartz, M Gnecchi
Stem Cell Res, 2018;29(0):170-173.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
J Neuroinflammation, 2018;15(1):226.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell line (UKWNLi001-A) from skin fibroblasts of a woman with Fabry disease carrying the X-chromosomal heterozygous c.708?G?>?C (W236C) missense mutation in exon 5 of the alpha-galactosidase-A gene
Authors: T Klein, K Günther, CK Kwok, F Edenhofer, N Üçeyler
Stem Cell Res, 2018;31(0):222-226.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells
Authors: M Jeong, M O'Reilly, NK Kirkwood, J Al-Aama, M Lako, CJ Kros, L Armstrong
Cell Death Dis, 2018;9(9):922.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Generation of two induced pluripotent stem cells lines from a Mucopolysaccharydosis IIIB (MPSIIIB) patient
Authors: S Vallejo-Di, A Fleischer, JM Martín-Fer, A Sánchez-Gi, D Bachiller
Stem Cell Res, 2018;33(0):180-184.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation
Authors: CP Cui, Y Zhang, C Wang, F Yuan, H Li, Y Yao, Y Chen, C Li, W Wei, CH Liu, F He, Y Liu, L Zhang
Nat Commun, 2018;9(1):4648.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an induced pluripotent stem cell line (TRNDi002-B) from a patient carrying compound heterozygous p.Q208X and p.G310G mutations in the NGLY1 gene
Authors: R Li, M Pradhan, M Xu, A Baskfield, A Farkhondeh, YS Cheng, J Beers, J Zou, C Liu, M Might, S Rodems, W Zheng
Stem Cell Res, 2018;34(0):101362.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an induced pluripotent stem cell line (TRNDi003-A) from a Noonan syndrome with multiple lentigines (NSML) patient carrying a p.Q510P mutation in the PTPN11 gene
Authors: R Li, A Baskfield, Y Lin, J Beers, J Zou, C Liu, F Jaffré, AE Roberts, EA Ottinger, MI Kontaridis, W Zheng
Stem Cell Res, 2018;34(0):101374.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice
Authors: O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour
Stem Cell Reports, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Central and Peripheral Nervous System Progenitors Derived from Human Pluripotent Stem Cells Reveal a Unique Temporal and Cell-Type Specific Expression of PMCAs
Authors: M Chen, SH Laursen, M Habekost, CH Knudsen, SH Buchholdt, J Huang, F Xu, X Liu, L Bolund, Y Luo, P Nissen, F Febbraro, M Denham
Front Cell Dev Biol, 2018;6(0):5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi002-A from a patient affected by the Jervell and Lange-Nielsen syndrome and carrier of two compound heterozygous mutations on the KCNQ1 gene
Authors: M Mura, YK Lee, M Ginevrino, R Zappatore, F Pisano, M Boni, F Dagradi, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi
Stem Cell Res, 2018;29(0):157-161.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The non-canonical Wnt-PCP pathway shapes the caudal neural plate
Authors: B López-Esco, JM Caro-Vega, DS Vijayragha, TF Plageman, JA Sanchez-Al, RC Moreno, D Savery, J Márquez-Ri, LA Davidson, P Ybot-Gonzá
Development, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Genome Editing in Neuroepithelial Stem Cells to Generate Human Neurons with High Adenosine-Releasing Capacity
Authors: D Poppe, J Doerr, M Schneider, R Wilkens, JA Steinbeck, J Ladewig, A Tam, DE Paschon, PD Gregory, A Reik, CE Müller, P Koch, O Brüstle
Stem Cells Transl Med, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation
Authors: M Naumann, A Pal, A Goswami, X Lojewski, J Japtok, A Vehlow, M Naujock, R Günther, M Jin, N Stanslowsk, P Reinhardt, J Sternecker, M Frickenhau, F Pan-Montoj, E Storkebaum, I Poser, A Freischmid, JH Weishaupt, K Holzmann, D Troost, AC Ludolph, TM Boeckers, S Liebau, S Petri, N Cordes, AA Hyman, F Wegner, SW Grill, J Weis, A Storch, A Hermann
Nat Commun, 2018;9(1):335.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Characterization of calcium signals in human induced pluripotent stem cell-derived dentate gyrus neuronal progenitors and mature neurons, stably expressing an advanced calcium indicator protein
Authors: G V?fély, T Berecz, E Szabó, K Szebényi, E Hathy, TI Orbán, B Sarkadi, L Homolya, MC Marchetto, JM Réthelyi, Á Apáti
Mol. Cell. Neurosci., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation
Authors: S Kwak, TW Kim, BH Kang, JH Kim, JS Lee, HT Lee, IY Hwang, J Shin, JH Lee, EJ Cho, HD Youn
Nucleic Acids Res., 2018;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions
Authors: A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra
Mol. Syst. Biol., 2018;14(1):e7952.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
TFAP2C regulates transcription in human naive pluripotency by opening enhancers
Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
Nat. Cell Biol., 2018;20(5):553-564.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
BMC Cancer, 2017;17(1):178.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A Core Regulatory Circuit in Glioblastoma Stem Cells Links MAPK Activation to a Transcriptional Program of Neural Stem Cell Identity
Authors: G Riddick, S Kotliarova, V Rodriguez, HS Kim, A Linkous, AJ Storaska, S Ahn, J Walling, G Belova, HA Fine
Sci Rep, 2017;7(0):43605.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
PLoS ONE, 2017;12(2):e0171947.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma
Authors: R Bayat Mokh, N Baluch, M Ka Hon Tsu, S Kumar, T S Homayoun, K Aitken, B Das, S Baruchel, H Yeger
BMC Cancer, 2017;17(1):156.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
Cell Death Discov, 2017;3(0):16105.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
Oncotarget, 2017;8(17):28074-28092.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding
Authors: S Falk, S Bugeon, J Ninkovic, GA Pilz, MP Postiglion, H Cremer, JA Knoblich, M Götz
Neuron, 2017;93(4):777-791.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
Stem Cell Res, 2017;21(0):58-70.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
Circ. Res, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression
Authors: AG Alvarado, PS Thiagaraja, EE Mulkearns-, DJ Silver, JS Hale, TJ Alban, SM Turaga, A Jarrar, O Reizes, MS Longworth, MA Vogelbaum, JD Lathia
Cell Stem Cell, 2017;20(4):450-461.e4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Repositioning CEP-1347, a chemical agent originally developed for the treatment of Parkinson's disease, as an anti-cancer stem cell drug
Authors: M Okada, H Takeda, H Sakaki, K Kuramoto, S Suzuki, T Sanomachi, K Togashi, S Seino, C Kitanaka
Oncotarget, 2017;8(55):94872-94882.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix
Authors: G Kaufman, D Skrtic
Tissue Cell, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1
Authors: K Hirano, M Namihira
FEBS Open Bio, 2017;7(12):1932-1942.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li
Stem Cell Res, 2017;22(0):29-32.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
Stem Cell Reports, 2017;8(1):125-139.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Laminin ?5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro
Authors: A Hyysalo, M Ristola, ME Mäkinen, S Häyrynen, M Nykter, S Narkilahti
Stem Cell Res, 2017;24(0):118-127.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Nicotinamide metabolism regulates glioblastoma stem cell maintenance
Authors: J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich
JCI Insight, 2017;2(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Purine synthesis promotes maintenance of brain tumor initiating cells in glioma
Authors: X Wang, K Yang, Q Xie, Q Wu, SC Mack, Y Shi, LJ Kim, BC Prager, WA Flavahan, X Liu, M Singer, CG Hubert, TE Miller, W Zhou, Z Huang, X Fang, A Regev, ML Suvà, TH Hwang, JW Locasale, S Bao, JN Rich
Nat. Neurosci, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
Sci Rep, 2016;6(0):30742.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
PLoS Biol., 2016;14(11):e1002581.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
PLoS ONE, 2016;11(5):e0155106.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
Stem Cells, 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
Stem Cell Res, 2016;17(2):441-443.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Enhancing the Three-Dimensional Structure of Adherent Gingival Fibroblasts and Spheroids via a Fibrous Protein-Based Hydrogel Cover
Cells Tissues Organs (Print), 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
Authors: Annabelle Congras
Sci Rep, 2016;6(0):27059.
Species: Porcine
Sample Types: Whole Cells
Applications: IHC -
Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A
Stem Cells Transl Med, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
Stem Cell Res, 2016;17(2):430-432.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
ODZ1 allows glioblastoma to sustain invasiveness through a Myc-dependent transcriptional upregulation of RhoA
Oncogene, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
EphA2 promotes infiltrative invasion of glioma stem cells in vivo through cross-talk with Akt and regulates stem cell properties.
Authors: Miao, H, Gale, N W, Guo, H, Qian, J, Petty, A, Kaspar, J, Murphy, A J, Valenzuela, D M, Yancopoulos, G, Hambardzumyan, D, Lathia, J D, Rich, J N, Lee, J, Wang, B
Oncogene, 2015;34(5):558-67.
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Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
Development, 2015;142(9):1628-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.
Authors: Kim, Dohoon, Fiske, Brian P, Birsoy, Kivanc, Freinkman, Elizavet, Kami, Kenjiro, Possemato, Richard, Chudnovsky, Yakov, Pacold, Michael, Chen, Walter W, Cantor, Jason R, Shelton, Laura M, Gui, Dan Y, Kwon, Manjae, Ramkissoon, Shakti H, Ligon, Keith L, Kang, Seong Wo, Snuderl, Matija, Vander Heiden, Matthew, Sabatini, David M
Nature, 2015;520(7547):363-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.
Authors: Iacopino F, Angelucci C, Piacentini R, Biamonte F, Mangiola A, Maira G, Grassi C, Sica G
PLoS ONE, 2014;9(8):e105166.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape.
Authors: Sherwood, Richard, Hashimoto, Tatsunor, O'Donnell, Charles, Lewis, Sophia, Barkal, Amira A, van Hoff, John Pet, Karun, Vivek, Jaakkola, Tommi, Gifford, David K
Nat Biotechnol, 2014;32(2):171-8.
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The subventricular zone is able to respond to a demyelinating lesion after localized radiation.
Authors: Capilla-Gonzalez V, Guerrero-Cazares H, Bonsu J, Gonzalez-Perez O, Achanta P, Wong J, Garcia-Verdugo J, Quinones-Hinojosa A
Stem Cells, 2014;32(1):59-69.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
Genes Dev, 2014;28(10):1085-100.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A single-cell and feeder-free culture system for monkey embryonic stem cells.
Authors: Ono T, Suzuki Y, Kato Y, Fujita R, Araki T, Yamashita T, Kato H, Torii R, Sato N
PLoS ONE, 2014;9(2):e88346.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: ICC -
Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex.
Authors: Minamide, Ryohei, Fujiwara, Kazushir, Hasegawa, Koichi, Yoshikawa, Kazuaki
PLoS ONE, 2014;9(1):e84460.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
J Immunol, 2014;192(1):523-32.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
Authors: Onishi K, Tonge P, Nagy A, Zandstra P
Stem Cell Reports, 2014;3(1):156-68.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Fluoxetine induces proliferation and inhibits differentiation of hypothalamic neuroprogenitor cells in vitro.
Authors: Sousa-Ferreira L, Aveleira C, Botelho M, Alvaro A, Pereira de Almeida L, Cavadas C
PLoS ONE, 2014;9(3):e88917.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Zrf1 is required to establish and maintain neural progenitor identity.
Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
Genes Dev, 2014;28(2):182-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi
Sci Rep, 2014;4(0):3701.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Fgf10-expressing tanycytes add new neurons to the appetite/energy-balance regulating centers of the postnatal and adult hypothalamus.
Authors: Haan, Niels, Goodman, Timothy, Najdi-Samiei, Alaleh, Stratford, Christin, Rice, Ritva, El Agha, Elie, Bellusci, Saverio, Hajihosseini, Mohammad
J Neurosci, 2013;33(14):6170-80.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
Nat Biotechnol, 2013;31(5):426-33.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Embryonic stem cell-based modeling of tau pathology in human neurons.
Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
Am J Pathol, 2013;182(5):1769-79.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Targeted deletion of the ERK5 MAP kinase impairs neuronal differentiation, migration, and survival during adult neurogenesis in the olfactory bulb.
Authors: Li, Tan, Pan, Yung-Wei, Wang, Wenbin, Abel, Glen, Zou, Junhui, Xu, Lihong, Storm, Daniel R, Xia, Zhengui
PLoS ONE, 2013;8(4):e61948.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
PLoS ONE, 2012;7(9):e45435.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
REST regulates oncogenic properties of glioblastoma stem cells.
Authors: Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H, Fuller GN, Lang FF, Majumder S
Stem Cells, 2012;30(3):405-14.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.
Authors: Lathia JD, Gallagher J, Myers JT
PLoS ONE, 2011;6(9):e24807.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Subchronic peripheral neuregulin-1 increases ventral hippocampal neurogenesis and induces antidepressant-like effects.
Authors: Mahar I, Tan S, Davoli MA, Dominguez-Lopez S, Qiang C, Rachalski A, Turecki G, Mechawar N
PLoS ONE, 2011;6(10):e26610.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel.
Authors: Hibino N, Villalona G, Pietris N
FASEB J., 2011;25(8):2731-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
Mol. Ther., 2011;19(3):584-93.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
Stem Cells Dev., 2011;20(6):1079-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Paternal recognition of adult offspring mediated by newly generated CNS neurons.
Authors: Mak GK, Weiss S,
Nat. Neurosci., 2010;13(6):753-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Diagnostic utility of novel stem cell markers SALL4, OCT4, NANOG, SOX2, UTF1, and TCL1 in primary mediastinal germ cell tumors.
Authors: Liu A, Cheng L, Du J, Peng Y, Allan RW, Wei L, Li J, Cao D
Am. J. Surg. Pathol., 2010;34(5):697-706.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Epigenetic changes to human umbilical cord blood cells cultured with three proteins indicate partial reprogramming to a pluripotent state.
Authors: Wong CJ, Casper RF, Rogers IM
Exp. Cell Res., 2010;316(6):927-39.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Noninvasive imaging of endogenous neural stem cell mobilization in vivo using positron emission tomography.
Authors: Rueger MA, Backes H, Walberer M, Neumaier B, Ullrich R, Simard ML, Emig B, Fink GR, Hoehn M, Graf R, Schroeter M
J. Neurosci., 2010;30(18):6454-60.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
Authors: Neganova I, Zhang X, Atkinson S, Lako M
Oncogene, 2009;28(1):20-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
J. Cell. Biochem., 2009;106(5):867-77.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures.
Authors: Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD
Stem Cells, 2008;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
Nat. Cell Biol., 2008;10(7):837-48.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation.
Authors: Lathia JD, Okun E, Tang SC, Griffioen K, Cheng A, Mughal MR, Laryea G, Selvaraj PK, Ffrench-Constant C, Magnus T, Arumugam TV, Mattson MP
J. Neurosci., 2008;28(51):13978-84.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Identification of SOX2 as a novel glioma-associated antigen and potential target for T cell-based immunotherapy.
Authors: Schmitz M, Temme A, Senner V, Ebner R, Schwind S, Stevanovic S, Wehner R, Schackert G, Schackert HK, Fussel M, Bachmann M, Rieber EP, Weigle B
Br. J. Cancer, 2007;96(8):1293-301.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
Stem Cells, 2007;25(5):1298-306.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
Stem Cells, 2007;25(6):1511-20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria.
Authors: Gunther HS, Schmidt NO, Phillips HS, Kemming D, Kharbanda S, Soriano R, Modrusan Z, Meissner H, Westphal M, Lamszus K
Oncogene, 2007;27(20):2897-909.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1.
Authors: Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M
Biochem. Biophys. Res. Commun., 2007;364(4):737-42.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
Stroke, 2006;37(9):2361-7.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
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Reviews for Human/Mouse/Rat SOX2 Antibody
Average Rating: 4.4 (Based on 7 Reviews)
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Cells: human induced pluripotent stem cells
Primary antibody: anti-human SOX2 (1:200), O/N incubation at 4 degrees
Secondary antibody: anti-mouse AF488
Red: sox2. Green: ki67. Blue: DAPI. Postnatal day 7 sub-ventricular zone. 2% PFA fixed. Heat-mediated antigen retrieval. Blocked with 10% donkey serum, 0.5% Triton in PBS for 1h. Dilution 1:100.
I used this antibody to test Sox2 expression in iPS cells derived from fibroblasts of Sanfilippo syndrome C patients.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 5% BSA in TBST
Dilution: 1/1000
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100