Recombinant Human p53 Protein, CF
Recombinant Human p53 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30 µM ZnCl2
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range of factors that promote DNA repair mechanisms or apoptosis, including cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).
- Arrowsmith, C.H. et al. (1999) Cell Death Differ. 6:1169.
- Dötsch, V. et al. (2010) Cold Spring Harb. Perspect. Biol. 2:a004887.
- Freed-Pastor, W.A. & C. Prives (2012) Genes Dev. 26:1268.
- Feng, Z. et al. (2011) J. Mol. Cell Biol. 3:44.
- Gu, B. & W.-G. Zhu (2012) Int. J. Biol. Sci. 8:672.
- Momand, J. et al. (1992) Cell 69:1237.
- Haupt, Y. et al. (1997) Nature 387:296.
Citations for Recombinant Human p53 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Regulators of Salmonella-host interaction identified by peripheral blood transcriptome profiling: roles of TGFB1 and TRP53 in intracellular Salmonella replication in pigs
Authors: T Huang, X Huang, B Shi, F Wang, W Feng, M Yao
Vet. Res., 2018;49(1):121.
Sample Types: Plasma
Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner
Authors: JJ Wamsley, C Gary, A Biktasova, M Hajek, G Bellinger, R Virk, N Issaeva, WG Yarbrough
Sample Types: Recombinant Protein
Applications: Enzyme Assay
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