Recombinant Human p53 Protein, CF

Catalog # Availability Size / Price Qty
SP-454-020
Product Details
Citations (2)
FAQs
Reviews

Recombinant Human p53 Protein, CF Summary

Purity
>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human p53 is ideal for use as a control substrate for in vitro Ubiquitin conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human p53 concentration of 0.5-2.5 μM.
Source
E. coli-derived human p53 protein
Accession #
Predicted Molecular Mass
44 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-454

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30 µM ZnCl2

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p53

p53 is well known for its key role as a tumor suppressor protein.  It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range of factors that promote DNA repair mechanisms or apoptosis, including  cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).

References
  1. Arrowsmith, C.H. et al. (1999) Cell Death Differ. 6:1169.
  2. Dötsch, V. et al. (2010) Cold Spring Harb. Perspect. Biol. 2:a004887.
  3. Freed-Pastor, W.A. & C. Prives (2012) Genes Dev. 26:1268.
  4. Feng, Z. et al. (2011) J. Mol. Cell Biol. 3:44.
  5. Gu, B. & W.-G. Zhu (2012) Int. J. Biol. Sci. 8:672.
  6. Momand, J. et al. (1992) Cell 69:1237.
  7. Haupt, Y. et al. (1997) Nature 387:296.
Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

Citations for Recombinant Human p53 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulators of Salmonella-host interaction identified by peripheral blood transcriptome profiling: roles of TGFB1 and TRP53 in intracellular Salmonella replication in pigs
    Authors: T Huang, X Huang, B Shi, F Wang, W Feng, M Yao
    Vet. Res., 2018;49(1):121.
    Species: Porcine
    Sample Types: Plasma
    Applications: Bioassay
  2. Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner
    Authors: JJ Wamsley, C Gary, A Biktasova, M Hajek, G Bellinger, R Virk, N Issaeva, WG Yarbrough
    Oncogenesis, 2017;6(4):e314.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay

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