Recombinant Human p53 Protein, CF

Name: Recombinant Human p53 Protein, CF
Brand: R&D Systems
SKU: SP-454

R&D Systems | Catalog # SP-454

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Citations (4)

Product Documents

Discontinued Product

SP-454 has been discontinued. View all p53 products.

Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Source

E. coli

Accession Number

P04637

Applications

Bioactivity

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Product Specifications

Source

E. coli-derived human p53 protein

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

44 kDa

Activity

Recombinant Human p53 is ideal for use as a control substrate for in vitro Ubiquitin conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human p53 concentration of 0.5-2.5 μM.

Formulation, Preparation, and Storage

SP-454

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30 µM ZnCl₂
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Background: p53

p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range of factors that promote DNA repair mechanisms or apoptosis, including cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).