Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus

(19 citations)
(2 Reviews)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.05-0.25 µg/mL.
  • Source
    E. coli-derived Cys25-Gly198 (Cys25Ile-Ile), with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    19.8 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
464-SH
 
464-SH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.05-0.25 μg/mL.
1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  • References:
    1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
    2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
    3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
    4. Echelard, Y. et al. (1993) Cell 75:1417.
    5. Zeng, X. et al. (2001) Nature 411:716.
    6. Feng, J. et al. (2004) Development 131:4357.
    7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
    8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
    9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
    10. Etheridge, L.A. et al. (2010) Development 137:133.
    11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
    12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
    13. Lewis, P.M. et al. (2001) Cell 105:599.
    14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
    15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
    16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
  • Entrez Gene IDs:
    6469 (Human); 20423 (Mouse)
  • Alternate Names:
    HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration
    Authors: Y Xiang, Y Tanaka, B Patterson, YJ Kang, G Govindaiah, N Roselaar, B Cakir, KY Kim, AP Lombroso, SM Hwang, M Zhong, EG Stanley, AG Elefanty, JR Naegele, SH Lee, SM Weissman, IH Park
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017;15(8):e2002063.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Explant culture of adult zebrafish hearts for epicardial regeneration studies
    Authors: J Cao, KD Poss
    Nat Protoc, 2016;11(5):872-81.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: Bioassay
  5. BarTeL, a Genetically Versatile, Bioluminescent and Granule Neuron Precursor-Targeted Mouse Model for Medulloblastoma
    Authors: Gregory M Shacklefor
    PLoS ONE, 2016;11(6):e0156907.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  6. Gli2 gene dosage and gene-environment interaction illuminate the etiological complexity of holoprosencephaly
    Dis Model Mech, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling.
    Authors: Wang J, Cao J, Dickson A, Poss K
    Nature, 2015;522(7555):226-30.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: Bioassay
  8. Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation.
    Authors: Shi Y, Chen J, Karner C, Long F
    Proc Natl Acad Sci U S A, 2015;112(15):4678-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015;6(0):6500.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Impact of the Smoothened inhibitor, IPI-926, on smoothened ciliary localization and Hedgehog pathway activity.
    Authors: Peluso, Marisa O, Campbell, Veronica, Harari, Joseph A, Tibbitts, Thomas T, Proctor, Jennifer, Whitebread, Nigel, Conley, James M, White, Kerry F, Kutok, Jeffery, Read, Margaret, McGovern, Karen, Faia, Kerrie L
    PLoS ONE, 2014;9(3):e90534.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells.
    Authors: Aguila J, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, Gayosso M, Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag K, Sanchez-Pernaute R
    Stem Cells Transl Med, 2014;3(9):1032-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Bax deficiency prolongs cerebellar neurogenesis, accelerates medulloblastoma formation and paradoxically increases both malignancy and differentiation.
    Authors: Garcia, I, Crowther, A J, Gama, V, Miller, C Ryan, Deshmukh, M, Gershon, T R
    Oncogene, 2013;32(18):2304-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
    Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
    Cell Stem Cell, 2013;12(5):559-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Primary cilia attenuate hedgehog signalling in neoplastic chondrocytes.
    Authors: Ho, L, Ali, S A, Al-Jazrawe, M, Kandel, R, Wunder, J S, Alman, B A
    Oncogene, 2013;32(47):5388-96.
    Species: Human
    Sample Type: Whole Tissue
    Application: tissue culture
  16. Specification of transplantable astroglial subtypes from human pluripotent stem cells.
    Authors: Krencik R, Weick JP, Liu Y, Zhang ZJ, Zhang SC
    Nat. Biotechnol., 2011;29(6):528-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
    Authors: Zeng H, Guo M, Martins-Taylor K
    PLoS ONE, 2010;5(7):e11853.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation.
    Authors: Nolan-Stevaux O, Lau J, Truitt ML, Chu GC, Hebrok M, Fernandez-Zapico ME, Hanahan D
    Genes Dev., 2009;23(1):24-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Levels of Gli3 repressor correlate with Bmp4 expression and apoptosis during limb development.
    Authors: Bastida MF, Delgado MD, Wang B, Fallon JF, Fernandez-Teran M, Ros MA
    Dev. Dyn., 2004;231(1):148-60.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 19 Citations
Average Rating: 5 (Based on 2 reviews)

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We have 2 review tested in 1 application: Stem/Immune cell maintenance or differentiation .
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 Stem/Immune cell maintenance or differentiation Anonymous 05/22/2017
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Reason for RatingUsed for patterning of iPSCs into neurons. Worked well.
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