Human/Mouse Tyrosine Hydroxylase Antibody

Catalog # Availability Size / Price Qty
MAB7566
MAB7566-SP
Human/Mouse Tyrosine Hydroxylase Antibody in Western Blot
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse Tyrosine Hydroxylase Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Tyrosine Hydroxylase in direct ELISAs and human, mouse and rat Tyrosine Hydroxylase in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tryptophan Hydroxylase-1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 779427
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Tyrosine Hydroxylase
Ala278-Tyr401
Accession # P07101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Tyrosine Hydroxylase by Western Blot. View Larger

Detection of Human, Mouse, and Rat Tyrosine Hydroxylase by Western Blot. Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Tyrosine Hydroxylase at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Tyrosine Hydroxylase in Mouse Dopaminergic Neurons. View Larger

Tyrosine Hydroxylase in Mouse Dopaminergic Neurons. Tyrosine Hydroxylase was detected in immersion fixed mouse embryonic stem cells differentiated into dopaminergic neurons using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Cells were double stained using the Northern-Lights™ 637-conjugated Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (white; Catalog # NL1195V). Cells were counterstained with DAPI (blue). Specific staining of Tyrosine Hydroxylase was localized to cytoplasm of dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Tyrosine Hydroxylase in Human Brain. View Larger

Tyrosine Hydroxylase in Human Brain. Tyrosine Hydroxylase was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse Tyrosine Hydroxylase by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse Tyrosine Hydroxylase by Simple WesternTM. Simple Western lane view shows lysates of human adrenal gland tissue and mouse adrenal gland tissue, loaded at 0.2 mg/mL. Specific bands were detected for Tyrosine Hydroxylase at approximately 52-61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyrosine Hydroxylase

TH (Tyrosine 3-hydroxylase), also known as Tyrosine 3-monooxygenase, is a 60-62 kDa member of the biopterin-dependent aromatic amino acid hydroxylase family of molecules. It is expressed by neurons of the dopamine and autonomic nervous system, plus the neuroendocrine cells of the adrenal medulla. TH is considered the rate limiting enzyme for catecholamine synthesis, and serves to catalyze the hydroxylation of L-tyrosine. It maintains stores of catecholamines following secretion, and its activity is regulated by targeted site phosphorylation. Human TH is 528 amino acids (aa) in length. It contains an N-terminal ACT domain (aa 69-190) that binds small molecules and regulates enzyme activity, and a C-terminal enzymatic region (aa 196-493). There are three significant utilized phosphorylation sites. Two at Ser31 and Ser40 increase enzyme activity, while phosphorylation at Ser19 promotes subsequent Ser40 phosphorylation. TH functions as a 240 kDa noncovalent homotetramer. There are four potential splice variants. One shows a deletion of aa 31-34, a second shows a deletion of aa 31-61, while a third contains a Met substitution for aa 1-34. A fourth isoform variant shows a deletion of aa 35-61. Over aa 278-401, human TH shares 94% aa sequence identity with mouse TH, a molecule that most closely resembles the fourth human isoform variant described above.

Entrez Gene IDs
7054 (Human); 21823 (Mouse); 25085 (Rat)
Alternate Names
DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase

Product Datasheets

Citations for Human/Mouse Tyrosine Hydroxylase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Upregulation of neuronal astrocyte elevated gene-1 protects nigral dopaminergic neurons in vivo
    Authors: E Leem, HJ Kim, M Choi, S Kim, YS Oh, KJ Lee, YS Choe, JY Um, WH Shin, JY Jeong, BK Jin, DW Kim, C McLean, PB Fisher, N Kholodilov, KS Ahn, JM Lee, UJ Jung, SG Lee, SR Kim
    Cell Death Dis, 2018;9(5):449.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Single-Factor SOX2 Mediates Direct Neural Reprogramming of Human Mesenchymal Stem Cells via Transfection of In Vitro Transcribed mRNA
    Authors: BE Kim, SW Choi, JH Shin, JJ Kim, I Kang, BC Lee, JY Lee, MG Kook, KS Kang
    Cell Transplant, 2018;0(0):9636897187718.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. A single cell high content assay detects mitochondrial dysfunction in iPSC-derived neurons with mutations in SNCA
    Authors: D Little, C Luft, O Mosaku, M Lorvellec, Z Yao, S Paillusson, J Kriston-Vi, S Gandhi, AY Abramov, R Ketteler, MJ Devine, P Gissen
    Sci Rep, 2018;8(1):9033.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. 27-Hydroxycholesterol increases ?-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons
    Authors: J Schommer, G Marwarha, T Schommer, T Flick, J Lund, O Ghribi
    BMC Neurosci, 2018;19(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Tyrosine Hydroxylase Antibody

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Human/Mouse Tyrosine Hydroxylase Antibody
By Anonymous on 10/27/2017
Application: ICC/IF Sample Tested: Sympathetic nerve,conjunctival sympathetic nerve Species: Mouse

Human/Mouse Tyrosine Hydroxylase Antibody
By Anonymous on 10/24/2016
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

TH positive neurons in red, co-stained with TUJ1 to mark neurons.