STAT3 Antibody (CL0490) - BSA Free

Name: STAT3 Antibody (CL0490) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52935
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52935

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL0490

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P40763, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for STAT3 Antibody (CL0490) - BSA Free

Western Blot: STAT3 Antibody (CL0490) [NBP2-52935]

Western Blot: STAT3 Antibody (CL0490) [NBP2-52935] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935]

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935] - Staining of human stomach cancer shows strong nuclear and moderate cytoplasmic positivity in tumor cells.

Western Blot: STAT3 Antibody (CL0490) [NBP2-52935]

Western Blot: STAT3 Antibody (CL0490) [NBP2-52935] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935]

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935] - Staining of human kidney shows strong nuclear combined with moderate cytoplasmic immunoreactivity.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935]

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935] - Staining of human prostate cancer shows strong nuclear and moderate cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935]

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0490) [NBP2-52935] - Staining of human rectum shows positivity in glandular cells.

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Applications for STAT3 Antibody (CL0490) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 Antibody (CL0490) - BSA Free

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Product Specific Notices for STAT3 Antibody (CL0490) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.