STAT3 Antibody (CL0490) - Azide and BSA Free

Name: STAT3 Antibody (CL0490) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-43940
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-43940

Submit a review

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL0490

Format

Azide and BSA Free

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P40763, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Novus Biologicals Mouse STAT3 Antibody (CL0490) - Azide and BSA Free (NBP2-52935) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT3 Antibody (CL0490) - Azide and BSA Free

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43940]

Analysis in human prostate and pancreas tissues using NBP3-43940 antibody. Corresponding STAT3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43940]

Staining of human kidney shows moderate to strong nuclear and cytoplasmic positivity in cells in glomeruli and tubuli.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43940]

Staining of human prostate shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43940]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43940]

Staining of human stomach cancer shows moderate to strong nuclear and cytoplasmic positivity in a subset of tumor cells.

Western Blot: STAT3 Antibody [NBP3-43940]

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

View 3 more images

Applications for STAT3 Antibody (CL0490) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 Antibody (CL0490) - Azide and BSA Free

Download Product Datasheets

Download SDS

Product Specific Notices for STAT3 Antibody (CL0490) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.