STAT3 [p Tyr705] Antibody

Name: STAT3 [p Tyr705] Antibody
Brand: Novus Biologicals
SKU: NB100-82213
Price: 269 USD
Availability: InStock
Rating: 4 (1 reviews)

Novus Biologicals | Catalog # NB100-82213

(1)

Citations (1)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).

Modification

p Tyr705

Specificity

Detects endogenous levels of STAT3 only when phosphorylated at tyrosine 705.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT3 [p Tyr705] Antibody

Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213]

Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213] - Analysis of extracts from Hela cells untreated or treated with IFN a using STAT3

Simple Western: STAT3 [p Tyr705] Antibody [NB100-82213]

Simple Western: STAT3 [p Tyr705] Antibody [NB100-82213] - STAT3-P assay on mouse megakaryocyte lysates. Simple Western image submitted by a verified customer review.

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] -

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] -

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.

Immunohistochemistry: STAT3 [p Tyr705] Antibody [NB100-82213] -

Immunohistochemistry: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunofluorescence analysis of mouse-spleen tissue.

View 2 more images

Applications for STAT3 [p Tyr705] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-200

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50 - 1:100

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:500-1:2000

Application Notes

This STAT3 [p Tyr705] Antibody is validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation

Reviewed Applications

Read 1 review rated 4 using NB100-82213 in the following applications:

Simple Western (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Gene Symbol

STAT3

UniProt

P40763

Additional STAT3 Products

Product Documents for STAT3 [p Tyr705] Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for STAT3 [p Tyr705] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Transcription Factors and Regulators

Dendritic Cell Development

GDNF Family

Interferons

Myeloid-derived Suppressor Cells (MDSC)

PDGF Family

Transcription Factors in the Akt Pathway

Citations for STAT3 [p Tyr705] Antibody

Customer Reviews for STAT3 [p Tyr705] Antibody (1)

4 out of 5

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STAT3 [p Tyr705] Antibody

Name: loubiere cécile

Application: Simple Western

Sample Tested: Megakaryocytes

Species: Mouse

Verified Customer | Posted 05/19/2021

STAT3-P assay on mouse megakaryocyte lysates

experience made on a Jess

Bio-Techne Response

This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.