TLR9 Antibody (26C593.2) - Azide and BSA Free

Name: TLR9 Antibody (26C593.2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-24863
Price: 539 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-24863

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Citations (53)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Monkey, Primate

Cited:

Human, Primate (Rhesus monkey)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Functional, In vitro assay, Knockdown Validated, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry Control, In vitro assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 26C593.2

Format

Azide and BSA Free

View all TLR9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).

Reactivity Notes

Rhesus Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for TLR9 Antibody (26C593.2) - Azide and BSA Free

Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]

Western Blot: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.

Flow (Intracellular): TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow (Intracellular): TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the PE conjugate of NBP2-24729. Staining of TLR9 in human PBMCs using 0.2 ug of this antibody. Shaded histogram represents cells without antibody; green represents a mouse IgG1-PE isotype control ; red represents anti-TLR9 antibody.

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Flow analysis of TLR9 in human PBMC using 0.5 ug of TLR9 antibody (red) and isotype control antibody (green).

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Intracellular flow cytometric analysis of TLR9 FITC in human B cells using 1ug/10^6 cells of NBP2-24908. Cells were primarily stained with PE-conjugated CD19 antibody. Cells were then fixed and permeabilized and stained with 1ug of either isotype control

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the Azide Free version of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the FITC conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Green represents isotype control ; red represents anti-TLR9 antibody. TLR intracellular flow kit was used for this test.

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Simple Western: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]

Simple Western: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Simple Western lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

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Applications for TLR9 Antibody (26C593.2) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:100 - 1:2000

Flow (Intracellular)

1:10 - 1:1000

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10 - 1:500

Simple Western

30 ug/ml

Western Blot

2-5 ug/ml

Application Notes

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17116765). Use in Immunohistochemistry reported in scientific literature (PMID: 16477017). Use in FLOW cell surface reported in scientific literature (PMID: 15879022).

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

Additional TLR9 Products

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Product Specific Notices for TLR9 Antibody (26C593.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.