Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Zebrafish Tie‑2 Fc Chimera (Catalog # 928-T2)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of Recombinant Human Angiopoietin-2 (Catalog # 623-AN) to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928‑T2) coated at 4 µg/mL (100 µL/well). At 0.5 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Angiopoietin-2 binding to Tie-2 blocked by Zebrafish Tie-2 Antibody. In a functional ELISA, Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928‑T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 μg/mL, this antibody will block 90% of the binding.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Zebrafish Tie-2 cDNA encodes a 1116 amino acid (aa) residue precursor protein shares 38% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
Takakura, N. et al. (1998) Immunity 9:677.
Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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