Bovine FGF basic/FGF2/bFGF Antibody

Catalog # Availability Size / Price Qty
Cell Proliferation Induced by FGF basic/FGF2/bFGF and Neutralization by Bovine FGF basic/FGF2/bFGF Antibody.
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Product Details
Citations (5)
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Bovine FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity
Detects bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with FGF acidic and recombinant human (rh)  beta -ECGF is observed and less than 1% cross-reactivity with rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed. Neutralizes the biological activity of bovine FGF basic/FGF2/bFGF and will also neutralize the biological activity of recombinant human FGF basic/FGF2/bFGF.
Polyclonal Rabbit IgG
Protein A or G purified
Bovine brain-derived FGF basic/FGF2/bFGF
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB)
Measured by its ability to neutralize FGF basic/FGF2/bFGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 1.5-4.5 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic/FGF2/bFGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF basic/FGF2/bFGF and Neutralization by Bovine FGF basic/FGF2/bFGF Antibody. View Larger

Cell Proliferation Induced by FGF basic/FGF2/bFGF and Neutralization by Bovine FGF basic/FGF2/bFGF Antibody. Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic/FGF2/bFGF/bFGF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF basic/FGF2/bFGF Polyclonal Antibody (Catalog # AB-33-NA). The ND50 is typically 1.5-4.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35‑55% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities.

FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

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Citations for Bovine FGF basic/FGF2/bFGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Impaired neural differentiation of MPS IIIA patient induced pluripotent stem cell-derived neural progenitor cells
    Authors: RJ Lehmann, LA Jolly, BV Johnson, MS Lord, HN Kim, JT Saville, M Fuller, S Byers, ALK Derrick-Ro
    Molecular genetics and metabolism reports, 2021-10-19;29(0):100811.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Maternal obesity and overnutrition alter fetal growth rate and cotyledonary vascularity and angiogenic factor expression in the ewe.
    Authors: Ma Y, Zhu MJ, Zhang L, Hein SM, Nathanielsz PW, Ford SP
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010-04-28;299(1):R249-58.
    Species: Ovine
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis.
    Authors: Lu J, Yang JH, Burns AR, Chen HH, Tang D, Walterscheid JP, Suzuki S, Yang CY, Sawamura T, Chen CH
    Circ. Res., 2009-01-15;104(5):619-27.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Dedicated epithelial recipient cells determine pigmentation patterns.
    Authors: Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL
    Cell, 2007-09-07;130(5):932-42.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003-07-02;23(13):5919-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization


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