Canine/Equine CD44 Antibody

Name: Canine/Equine CD44 Antibody
Brand: R&D Systems
SKU: MAB5449
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # MAB5449

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Citations (11)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Canine, Equine

Cited:

Canine, Equine

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 69-S5

View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Canine marrow cells
Accession # Q28284

Specificity

Detects canine and equine CD44 in Flow Cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Canine/Equine CD44 Antibody

Detection of CD44 in Canine Monocytes by Flow Cytometry.

Canine blood-derived monocytes were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Detection of CD44 in Equine PBMCs by Flow Cytometry.

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Equine CD44 by Flow Cytometry

Immunophenotyping and multipotency assay. Representative dot plots from flow cytometry analysis (A). Expression of surface antigens were investigated in cells cultured in control and AZA/RES supplemented medium. Isolated cells were characterized by the expression of CD90 and CD44 while lacked the expression of CD45 hematopoietic marker (B). Multipotency of isolated cells was confirmed by three lineage differentiation (C). In order to confirm formation of proteoglycans during chondrogenesis cells were stained with Safranin O. Intracellular lipid droplets were visualized by Oil Red O while mineralized matrix formed during osteogenesis with Alizarin Red. Magnification ×100, scale bars: 250 μm. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the result to ASCCTRL, and as hashtag (#) when comparing to ASCEMS. ###P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30370650), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Canine/Equine CD44 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Canine blood‑derived monocytes and equine peripheral blood mononuclear cells (PBMCs)

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD44

Canine CD44 is a ubiquitously expressed 85‑90 kDa transmembrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated by alternate splicing and variable posttranslational modification. Within the N‑terminal invariant portion of the ECD (aa 14‑191), canine CD44 shares 90%, 83%, and 82% identity with human, mouse, and rat CD44, respectively.