Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2577B
View all Digoxigenin Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Digoxigenin

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Digoxigenin Antibody

Digoxigenin antibody in Human Liver by Immunohistochemistry (IHC-P).

Digoxigenin in Human Liver.

Digoxigenin was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Digoxigenin labeled anti-Vimentin antibodies bound in the bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Digoxigenin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Digoxigenin in HeLa Human Cell Line.

Digoxigenin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm incubated with Digoxigenin-labeled anti-Vimentin antibody. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Digoxigenin Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and Digoxigenin labeled anti-Vimentin antibody

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and Digoxigenin labeled anti-Vimentin antibody

Reviewed Applications

Read 1 review rated 5 using MAB10386 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody

Certificate of Analysis

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Product Specific Notices for Digoxigenin Antibody

For research use only

Customer Reviews for Digoxigenin Antibody (1)

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  • Human Digoxigenin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 10/22/2022
    Digoxigenin Antibody MAB10386

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Protocols

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