Equine IL-2 Biotinylated Antibody

  
  • Species Reactivity
    Equine
  • Specificity
    Detects equine IL‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant bovine IL-2, recombinant canine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant equine IL‑2
    Ala21-Thr149 (Cys141Ser)
    Accession # NP_001078902.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Equine IL‑2 (Cys141Ser) (Catalog # 1613-IL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Equine IL-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Equine IL‑2 Antibody (Catalog # AF1613)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 Not Assigned 
    Equine IL‑2 Biotinylated Antibody (Catalog # BAF1613)
  • ELISA Standard
     
    Recombinant Equine IL-2 (Cys141Ser) Protein (Catalog # 1613-IL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑2 in Equine PBMCs. IL‑2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1613) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). IL-2 has since been found to also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes (2).

The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor that mediate IL-2 signals is composed of three polypeptide chains, the IL-2 receptor alpha, beta and gamma  subunits (3). IL-2 also signals via the intermediate affinity receptor complex of the beta  and gamma subunits (4). In T cells, the beta  and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-21 (6).

At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and mouse IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on mouse CTLL-2 cells (7).

  • References:
    1. Morgan, D.A. et al. (1976) Science 193:1007.
    2. Smith, K.A. et al. (1988) Science 240:1169.
    3. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
    4. Giri, J. et al. (1994) EMBO J. 13:2822.
    5. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
    6. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
    7. E.V. Vandergrift and D.W. Horohov (1993) Vet. Immunol. Immunopathol. 39:395.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
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