ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free

Name: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-14157
Price: 509 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14157

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # PCRP-MAPK1-1D1

Format

Azide and BSA Free

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ERK2 protein (Uniprot: P28482)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13754).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free

Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157]

Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157] -

Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Applications for ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

0.5-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Application Notes

ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.