ERK2 Antibody - BSA Free

Name: ERK2 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-87943
Price: 319 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-87943

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YIVQDLMETDLYKLLKTQHLSNDHICY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.

Simple Western: ERK2 Antibody [NBP1-87943]

Simple Western: ERK2 Antibody [NBP1-87943] - Simple Western lane view shows a specific band for ERK2 in 0.2 mg/ml of U-2 OS (left) and h. Pancreas (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: ERK2 Antibody [NBP1-87943]

Simple Western: ERK2 Antibody [NBP1-87943] - Electropherogram image of the corresponding Simple Western lane view. ERK2 antibody was used at 1:5 dilution on U-2 OS and h. Pancreas lysate(s) respectively.

Western Blot: ERK2 Antibody - BSA Free [NBP1-87943]

Analysis in human cell line HDLM-2.

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Applications for ERK2 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:5

Western Blot

1:100-1:250

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-2 OS and h pancreas lysates, separated by Size, antibody dilution of 1:5

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

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Certificate of Analysis

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Product Specific Notices for ERK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.