Feline IL-2 Biotinylated Antibody Summary
Accession # Q07885
Feline IL-2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑2 in Feline PBMCs. IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1890) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta and gamma subunits. The non-ligand binding gamma subunit, referred to as the common gamma subunit ( gamma c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8+ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8+ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1‑9).
- Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
- Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
- Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
- Matesanz, F. et al. (1993) Immunogenetics 38:300.
- Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
- Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
- Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
- Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
- Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Feline IL-2 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Feline IL-2 Biotinylated Antibody and earn rewards!
Have you used Feline IL-2 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image