Feline IL-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1890
Feline IL-2 Biotinylated Antibody in Data
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Feline IL-2 Biotinylated Antibody Summary

Species Reactivity
Feline
Specificity
Detects feline IL‑2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross‑reactivity with recombinant canine IL-2 is observed, approximately 1% cross-reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed, and less than 0.2% cross‑reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant feline IL‑2
Ala21-Thr154 (Cys146Ser)
Accession # Q07885
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Feline IL‑2 (Cys146Ser) (Catalog # 1890-FL)
Immunocytochemistry
5-15 µg/mL
See below

Feline IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Feline IL‑2 Antibody (Catalog # AF1890)

Standard: Recombinant Feline IL-2 (Cys146Ser) Protein (Catalog # 1890-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑2 in Feline PBMCs. View Larger

IL‑2 in Feline PBMCs. IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1890) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma  subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta  and gamma  subunits. The non-ligand binding   gamma  subunit, referred to as the common gamma  subunit ( gamma c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8+ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8+ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1‑9).

References
  1. Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
  2. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
  3. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
  8. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

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