Feline IL-2 Antibody

(1 citations)   
  • Species Reactivity
    Feline
  • Specificity
    Detects feline IL‑2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed, and less than 0.2% cross‑reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant feline IL‑2
    Ala21-Thr154 (Cys146Ser)
    Accession # Q07885
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Feline IL‑2 (Cys146Ser) (Catalog # 1890-FL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Feline IL-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Feline IL‑2 Antibody (Catalog # AF1890)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Feline IL‑2 Biotinylated Antibody (Catalog # BAF1890)
  • ELISA Standard
     
    Recombinant Feline IL-2 (Cys146Ser) Protein (Catalog # 1890-FL)
  • Neutralization
    Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A. J. H. and C. B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.2 ng/mL Recombinant Feline IL‑2 (Cys146Ser).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑2 and Neutralization by Feline IL‑2 Antibody. Recombinant Feline IL‑2 (Cys146Ser) (Catalog #
1890-FL) induces cell proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL‑2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND50 is typically
0.03-0.15 µg/mL.

Immunocytochemistry
IL‑2 in Feline PBMCs. IL‑2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2

Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be
O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma  subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta and gamma  subunits. The non-ligand binding   gamma  subunit, referred to as the common gamma  subunit ( gamma c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8+ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8+ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1-9).

  • References:
    1. Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
    2. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
    3. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
    4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
    5. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
    6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
    7. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
    8. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
    9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-alpha plasmids preserves global CD4 T lymphocyte function after challenge with FIV.
    Authors: Maksaereekul S, Dubie RA, Shen X, Kieu H, Dean GA, Sparger EE
    Vaccine, 2009;27(28):3754-65.
    Species: Feline
    Sample Type: Cell Culture Supernates
    Application: WB
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