Cell Proliferation Induced by IL‑2 and Neutralization by Feline IL‑2 Antibody. Recombinant Feline IL‑2 (Cys146Ser) (Catalog #|
1890-FL) induces cell proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL‑2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND50 is typically
|IL‑2 in Feline PBMCs. IL‑2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be
O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta and gamma subunits. The non-ligand binding gamma subunit, referred to as the common gamma subunit ( gamma c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8+ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8+ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1-9).
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