Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carboxylesterase 1/CES1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase 1/CES1
His19-Glu563
Accession # NP_001020365

Specificity

Detects human Carboxylesterase 1/CES1 in direct ELISAs and Western blots. Detects mouse Carboxylesterase 1/CES1 and rat Carboxylesterase 1/CES1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Detection of Human, Mouse, and Rat Carboxylesterase 1/CES1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Carboxylesterase 1/CES1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4920) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 1/CES1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse Carboxylesterase 1/CES1 antibody by Simple WesternTM.

Detection of Human and Mouse Carboxylesterase 1/CES1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carboxylesterase 1/CES1 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4920) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human liver tissue and mouse liver tissue

Western Blot

0.2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, human liver tissue, mouse liver tissue, and rat liver tissue

Reviewed Applications

Read 1 review rated 5 using AF4920 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxylesterase 1/CES1

Carboxylesterase 1 (CES1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES1 shares the serine hydrolase fold observed in other esterases (3). CES1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus.

References

  1. Redinbo, M.R. and P.M. Potter (2005) Drug Discovery Today 10:313.
  2. Satoh, T. and M. Hosokawa (2006) Chem. Biol. Interactions 162:195.
  3. Fleming, C.D. et al. (2007) Biochemistry 46:5603.

Alternate Names

ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH

Entrez Gene IDs

1066 (Human); 12623 (Mouse); 29225 (Rat)

Gene Symbol

CES1

UniProt

NP_001020365

Additional Carboxylesterase 1/CES1 Products

Product Documents for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

For research use only

Citations for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody

Customer Reviews for Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody (1)

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  • Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Caco-2 human colorectal adenocarcinoma cell line
    Species: cell lysate
    Verified Customer | Posted 02/17/2020
    Human/Mouse/Rat Carboxylesterase 1/CES1 Antibody AF4920

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