Human/Mouse/Rat Vimentin Antibody
Human/Mouse/Rat Vimentin Antibody Summary
Ser2-Glu466
Accession # P08670
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Vimentin by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, RAW 264.7 mouse monocyte/macrophage cell line, NR8383 rat alveolar macrophage cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Vimentin in HeLa Human Cell Line. Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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Vimentin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our Fluorescent ICC Staining of Non-adherent Cells protocol.
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Western Blot Shows Human Vimentin Specificity by Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vimentin
Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
- Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
- Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
- Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
- Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
- Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
- Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716.
- Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
- Styers, M.L. et al. (2005) Traffic 6:359.
- McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
- Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
- Ivaska, J. et al. (2005) EMBO J. 24:3834.
- Byun, Y. et al. (2001) Cell Death Differ. 8:443.
- Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
- Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
- Garg, A. et al. (2006) J. Immunol. 177:6192.
Product Datasheets
Citations for Human/Mouse/Rat Vimentin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL
Authors: Y Weng, H Wang, D Wu, S Xu, X Chen, J Huang, Y Feng, L Li, Z Wang
Cell Research, 2022;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
Nature Communications, 2022;13(1):5494.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A comprehensive library of human transcription factors for cell fate engineering
Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
Nature Biotechnology, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts
Authors: E Sugawara, M Kato, Y Kudo, W Lee, R Hisada, Y Fujieda, K Oku, T Bohgaki, O Amengual, S Yasuda, T Onodera, S Hatakeyama, T Atsumi
Autophagy, 2019;0(0):1-10.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
Mol Med Rep, 2018;0(0):.
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Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
Respir. Res., 2017;18(1):82.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
Authors: Lynne A Murray, Rebecca Dunmore, Ana Camelo, Carla A Da Silva, Malin J Gustavsso, David M Habiel, Tillie L Hackett, Cory M Hogaboam, Matthew A Sleeman, Darryl A Knight
Respiratory Research, 2017;0(0):1465-993X.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Dexamethasone mediates pancreatic cancer progression by glucocorticoid receptor, TGF? and JNK/AP-1
Authors: L Liu, E Aleksandro, F Schönsiege, D Gröner, N Bauer, CC Nwaeburu, Z Zhao, J Gladkich, T Hoppe-Tich, E Yefenof, T Hackert, O Strobel, I Herr
Cell Death Dis, 2017;8(10):e3064.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
Authors: Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
PLoS ONE, 2016;11(1):e0147155.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery
Authors: David G Gorenstein
BioTechniques, 2016;61(5):249-259.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay -
TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
PLoS ONE, 2016;11(9):e0162221.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The mosaic of "seronegative" antiphospholipid syndrome.
Authors: Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
J Immunol Res, 2014;2014(0):389601.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
Authors: Teo, Catherin, Chu, Justin J
J Virol, 2014;88(4):1897-913.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
PLoS ONE, 2012;7(7):e40490.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
Authors: Windus L, Kiss D, Glover T, Avery V
Exp Cell Res, 2012;318(19):2507-19.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
Authors: Lai D, Ma L, Wang F
Int. J. Oncol., 2012;41(2):541-50.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
Authors: Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
Blood, 2010;116(16):2960-7.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
Am. J. Physiol., Cell Physiol., 2010;298(5):C1217-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
PLoS ONE, 2010;5(9):e13002.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
Authors: Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA
Am. J. Respir. Crit. Care Med., 2009;180(2):122-33.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
Authors: Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung da W, Jeon NK, Choi YW, Lee EJ, Kim J
Cancer Lett., 2009;275(1):61-71.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
Int J Med Sci, 0;12(3):248-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
Authors: Mercurio L, Ajmone-Cat M, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
J Exp Clin Cancer Res, 0;35(0):55.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC
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