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Human/Mouse/Rat Vimentin Antibody

Catalog #: AF2105
24 Citations 7 Reviews Datasheet / COA / SDS
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AF2105
AF2105-SP
Detection of Human, Mouse, and Rat Vimentin by Western Blot.
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Product Details
Citations (24)
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Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Vimentin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Vimentin in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
Vimentin is specifically detected in K562 human chronic myelogenous leukemia cell line but is not detectable in Vimentin knockout K562 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Vimentin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Vimentin by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, RAW 264.7 mouse monocyte/macrophage cell line, NR8383 rat alveolar macrophage cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry Vimentin antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry Vimentin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Vimentin in HeLa Human Cell Line. Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Vimentin antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Knockout Validated Vimentin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

Vimentin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our Fluorescent ICC Staining of Non-adherent Cells protocol.

Knockout Validated Western Blot Shows Human Vimentin Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Vimentin Specificity by Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot Detection of Human Vimentin antibody by Western Blot. View Larger

Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

References
  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.
Entrez Gene IDs
7431 (Human); 22352 (Mouse); 81818 (Rat)
Alternate Names
epididymis secretory sperm binding protein; FLJ36605; VIM; Vimentin

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Citations for Human/Mouse/Rat Vimentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL
    Authors: Y Weng, H Wang, D Wu, S Xu, X Chen, J Huang, Y Feng, L Li, Z Wang
    Cell Research, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
    Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
    Nature Communications, 2022;13(1):5494.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A comprehensive library of human transcription factors for cell fate engineering
    Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
    Nature Biotechnology, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts
    Authors: E Sugawara, M Kato, Y Kudo, W Lee, R Hisada, Y Fujieda, K Oku, T Bohgaki, O Amengual, S Yasuda, T Onodera, S Hatakeyama, T Atsumi
    Autophagy, 2019;0(0):1-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
    Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
    Mol Med Rep, 2018;0(0):.
  7. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
    Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
    Respir. Res., 2017;18(1):82.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
    Authors: Lynne A Murray, Rebecca Dunmore, Ana Camelo, Carla A Da Silva, Malin J Gustavsso, David M Habiel, Tillie L Hackett, Cory M Hogaboam, Matthew A Sleeman, Darryl A Knight
    Respiratory Research, 2017;0(0):1465-993X.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Dexamethasone mediates pancreatic cancer progression by glucocorticoid receptor, TGF? and JNK/AP-1
    Authors: L Liu, E Aleksandro, F Schönsiege, D Gröner, N Bauer, CC Nwaeburu, Z Zhao, J Gladkich, T Hoppe-Tich, E Yefenof, T Hackert, O Strobel, I Herr
    Cell Death Dis, 2017;8(10):e3064.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
    Authors: Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
    PLoS ONE, 2016;11(1):e0147155.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  11. Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery
    Authors: David G Gorenstein
    BioTechniques, 2016;61(5):249-259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  12. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
    PLoS ONE, 2016;11(9):e0162221.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. The mosaic of "seronegative" antiphospholipid syndrome.
    Authors: Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
    J Immunol Res, 2014;2014(0):389601.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  14. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
    Authors: Teo, Catherin, Chu, Justin J
    J Virol, 2014;88(4):1897-913.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012;7(7):e40490.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
    Authors: Windus L, Kiss D, Glover T, Avery V
    Exp Cell Res, 2012;318(19):2507-19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
    Authors: Lai D, Ma L, Wang F
    Int. J. Oncol., 2012;41(2):541-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
    Authors: Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
    Blood, 2010;116(16):2960-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  19. Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
    Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
    Am. J. Physiol., Cell Physiol., 2010;298(5):C1217-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
    Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
    PLoS ONE, 2010;5(9):e13002.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
    Authors: Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA
    Am. J. Respir. Crit. Care Med., 2009;180(2):122-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
    Authors: Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung da W, Jeon NK, Choi YW, Lee EJ, Kim J
    Cancer Lett., 2009;275(1):61-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
    Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
    Int J Med Sci, 0;12(3):248-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
    Authors: Mercurio L, Ajmone-Cat M, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
    J Exp Clin Cancer Res, 0;35(0):55.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human/Mouse/Rat Vimentin Antibody

Average Rating: 3.7 (Based on 7 Reviews)

5 Star
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Human/Mouse/Rat Vimentin Antibody
By Anonymous on 12/20/2022
Application: Ab concentration 2 ug/mL, no specific band detected Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human
Other Human/Mouse/Rat Vimentin Antibody AF2105
Human/Mouse/Rat Vimentin Antibody
By Anonymous on 07/02/2021
Application: ICC/IF Sample Tested: Cancer Tissue Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Vimentin Antibody AF2105
Human/Mouse/Rat Vimentin Antibody
By Anonymous on 02/20/2021
Application: IHC Sample Tested: Pancreatic cancer tissue Species: Mouse
Immunohistochemistry Human/Mouse/Rat Vimentin Antibody AF2105
Human Vimentin Antibody
By Anonymous on 06/27/2018
Application: ICC/IF Sample Tested: Pancreas tissue Species: Mouse
Human Vimentin Antibody
By simon liu on 12/19/2017
Application: ICC/IF Sample Tested: Bladder tissue Species: Human

Technical Service will be following up

The customer followed up reporting the poor staining may have been caused by storing the product outside of our guarantee.

Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Human Vimentin Antibody
By Anonymous on 12/10/2017
Application: ICC/IF Sample Tested: Kidney cancer tissue Species: Human
Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Human Vimentin Antibody
By Juan Zanin on 07/22/2016
Application: ICC/IF Sample Tested: Cerebellum tissue Species: Mouse

P7 MOUSE CEREBELLUM. Radial Glia

Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105