GSK-3 beta Antibody (M131)

Name: GSK-3 beta Antibody (M131)
Brand: Novus Biologicals
SKU: NBP3-23366
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23366

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M131

View all GSK-3 beta Primary Antibodies »

Product Specifications

Immunogen

Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3 beta. This sequence is highly conserved in rat and mouse GSK-3 beta, and has 65% homology to similar regions in GSK-3 alpha. [UniProt# P49841]

Specificity

The antibody detects a 46 kDa protein corresponding to the apparent molecular mass of GSK-3 beta on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for GSK-3 beta Antibody (M131)

Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] -

Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] -

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] - Immunocytochemical labeling of GSK-3 beta in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK-3 beta (NBP3-23366) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for GSK-3 beta Antibody (M131)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:250

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GSK-3 beta

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Gene Symbol

GSK3B

Additional GSK-3 beta Products

Product Documents for GSK-3 beta Antibody (M131)

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Certificate of Analysis

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Product Specific Notices for GSK-3 beta Antibody (M131)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.