HA Tag Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB0601
Detection of HA-tagged proteins by Western Blot.
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HA Tag Antibody Summary

Specificity
Detects N-terminal and C-terminal HA-tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA Tag peptide
Accession # ABB51961
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
1-4 µg/100 µg cell lysate
HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, see our available Western blot detection antibodies.
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of HA-tagged proteins by Western Blot. View Larger

Detection of HA-tagged proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, C-terminal HA-tagged TRANCE, and
C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HA-tagged proteins at approximately 70 kDa, 40 kDa, and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HA Tag in NS0 Mouse Cell Line. View Larger

HA Tag in NS0 Mouse Cell Line. HA was detected in immersion fixed NS0 mouse myeloma cell line transfected with HA-tagged WISP-2 using Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of HA-tagged proteins by Simple WesternTM. View Larger

Detection of HA-tagged proteins by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with
C-terminal HA-tagged LRRN5 and
N-terminal HA-tagged MICA, loaded at 0.1 mg/mL. Specific bands were detected for HA-tagged proteins at approximately 87 and 106 kDa (as indicated) using 1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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