Human 15-PGDH/HPGD Antibody

Catalog # Availability Size / Price Qty
AF5660
AF5660-SP
Detection of Human 15-PGDH/HPGD by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human 15-PGDH/HPGD Antibody Summary

Species Reactivity
Human
Specificity
Detects human 15-PGDH/HPGD in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 15-PGDH/HPGD
Met1-Gln266
Accession # P15428
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human 15-PGDH/HPGD antibody by Western Blot. View Larger

Detection of Human 15-PGDH/HPGD by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for 15-PGDH/HPGD at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human 15-PGDH/HPGD antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human 15‑PGDH/HPGD by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for 15-PGDH/HPGD at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 15-PGDH/HPGD

HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD+-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).

Long Name
15-Hydroxyprostaglandin Dehydrogenase
Entrez Gene IDs
3248 (Human); 15446 (Mouse); 79242 (Rat)
Alternate Names
15-hydroxyprostaglandin dehydrogenase [NAD+]; 15PGDH; 15-PGDH; EC 1.1.1; EC 1.1.1.141; HPGD; hydroxyprostaglandin dehydrogenase 15-(NAD); NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; PGDH1; PGDH1PGDH; Prostaglandin dehydrogenase 1; SDR36C1; short chain dehydrogenase/reductase family 36C, member 1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human 15-PGDH/HPGD Antibody

There are currently no reviews for this product. Be the first to review Human 15-PGDH/HPGD Antibody and earn rewards!

Have you used Human 15-PGDH/HPGD Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review