Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 1014728
View all 5-HT1D Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human 5-HT1D
Accession # P28221

Specificity

Detects human 5-HT1D in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human 5-HT1D Antibody

5-HT1D antibody in Human Brain by Immunohistochemistry (IHC-P).

5-HT1D in Human Brain.

5-HT1D was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human 5-HT1D Monoclonal Antibody (Catalog # MAB10354) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of 5-HT1D in Rat Brain.

5-HT1D was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Mouse Anti-Human 5-HT1D Monoclonal Antibody (Catalog # MAB10354) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human 5-HT1D Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) tissue and perfusion fixed paraffin-embedded sections of Rat Brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-HT1D

Human 5-hydroxytryptamine receptor 1D, or 5-HT1D, is a G-protein coupled receptor for serotonin. 5-HT1D also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances. Ligand binding causes a conformation changes in a typical G-protein/adenylate cyclase fashion.

Long Name

5-Hydroxytryptamine Receptor 1D

Alternate Names

5HT1D, HT1DA, HTR1D, HTR1DA, HTRL, RDC4

Entrez Gene IDs

3352 (Human); 15552 (Mouse); 25323 (Rat)

Gene Symbol

HTR1D

UniProt

P28221 (Rat)

Additional 5-HT1D Products

Product Documents for Human 5-HT1D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 5-HT1D Antibody

For research use only

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Protocols

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