Human AATK Antibody

Catalog # Availability Size / Price Qty
Detection of AATK in A172 Human Cell Line by Flow Cytometry.
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Product Details
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Human AATK Antibody Summary

Species Reactivity
Detects human AATK in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 456006
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human AATK
Accession # AAH47378
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of AATK antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of AATK in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human AATK Monoclonal Antibody (Catalog # MAB4866, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AATK

Apoptosis-associated tyrosine kinase (AATK) is a 155 kDa membrane-associated intracellular protein that promotes neurite extension and neuronal differentiation. Low extracellular potassium levels induce AATK phosphorylation and apoptosis in cerebellar granule cells. AATK negatively regulates the Na-K-2Cl cotransporter by promoting its PP1-dependent dephosphorylation. The two alternate splice forms of AATK also contain the sequences included in this immunogen. Within this region, human AATK shares 63% aa sequence identity with mouse AATK.

Long Name
Apoptosis-associated Tyrosine Kinase
Entrez Gene IDs
9625 (Human); 11302 (Mouse); 690853 (Rat)
Alternate Names
AATK; AATYK; AATYKp35-binding protein; apoptosis-associated tyrosine kinaseCDK5-binding protein; Brain apoptosis-associated tyrosine kinase; EC 2.7.11; KIAA0641p35BP; Lemur tyrosine kinase 1; LMR1; LMR1EC; LMTK1; LMTK1AATYK1; p35BP; serine/threonine-protein kinase LMTK1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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