Human Activin RIA/ALK-2 Antibody Summary
Accession # Q04771
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Activin RIA/ALK‑2 in HUVEC Human Cells. Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Activin RIA/ALK-2
Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.
- Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
- Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
Citations for Human Activin RIA/ALK-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
Filter your results:
Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils
Authors: Y Kimishima, T Misaka, T Yokokawa, K Wada, K Ueda, K Sugimoto, K Minakawa, K Nakazato, T Ishida, M Oshima, S Koide, K Shide, K Shimoda, A Iwama, K Ikeda, Y Takeishi
Nature Communications, 2021;12(1):6177.
Species: Transgenic Human
Sample Types: Cell Lysates
Applications: Western Blot
Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate
Authors: HN Arai, F Sato, T Yamamoto, K Woltjen, H Kiyonari, Y Yoshimoto, C Shukunami, H Akiyama, R Kist, A Sehara-Fuj
Cell Rep, 2019;29(3):603-616.e5.
Sample Types: Whole Cells
Applications: Flow Cytometry
Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7.
Authors: Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH
Eur. J. Immunol., 2011;41(11):3135-45.
Sample Types: Whole Cells
Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
J. Biol. Chem., 2007;282(32):23337-47.
Sample Types: Whole Tissue
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Activin RIA/ALK-2 Antibody
Average Rating: 3 (Based on 1 Review)
Have you used Human Activin RIA/ALK-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
10ug of protein collected from untreated HEK, HUVEC, and HAEC cells was loaded into each well.
Blocked in Prometheus OneBlock Western-CL Blocking Buffer for 1hr @ room temp
Primary antibody: Anti-ALK2 MAB637, mouse, RnD 1:500 overnight @ 4C
Secondary antibody: goat-α-mouse HRP (Thermo) 1:10,000 1 hr @ room temp
Substrate: Luminata Forte Western HRP Substrate Millipore 2 min
Imaging: 300 sec exposure