Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 71412
View all Activin RIA/ALK-2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK‑2
Asp23-Val124
Accession # Q04771

Specificity

Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Activin RIA/ALK‑2 Antibody

Activin RIA/ALK-2 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

Activin RIA/ALK‑2 in HUVEC Human Cells.

Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Activin RIA/ALK‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human Activin RIA/ALK-2 Fc Chimera (Catalog # 637-AR)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 3 using MAB637 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIA/ALK-2

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

References

  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Long Name

Activin Receptor IA

Alternate Names

ActivinRIA, ACVR1, ALK-2, ALK2

Entrez Gene IDs

90 (Human); 11477 (Mouse)

Gene Symbol

ACVR1

UniProt

Q04771

Additional Activin RIA/ALK-2 Products

Product Documents for Human Activin RIA/ALK‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Activin RIA/ALK‑2 Antibody

For research use only

Citations for Human Activin RIA/ALK‑2 Antibody

Customer Reviews for Human Activin RIA/ALK‑2 Antibody (1)

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  • Human Activin RIA/ALK-2 Antibody
    Name: Molly Kulikauskas
    Application: Western Blot
    Sample Tested: HUVEC human umbilical vein endothelial cells, HAEC human aortic endothelial cells and HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 08/27/2018
    10ug of protein collected from untreated HEK, HUVEC, and HAEC cells was loaded into each well. Blocked in Prometheus OneBlock Western-CL Blocking Buffer for 1hr @ room temp Primary antibody: Anti-ALK2 MAB637, mouse, RnD 1:500 overnight @ 4C Secondary antibody: goat-alpha -mouse HRP (Thermo) 1:10,000 1 hr @ room temp Substrate: Luminata Forte Western HRP Substrate Millipore 2 min Imaging: 300 sec exposure
    Human Activin RIA/ALK‑2 Antibody MAB637

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