Human Activin RIA/ALK-2 Antibody

Catalog # Availability Size / Price Qty
MAB637-SP
MAB637-500
MAB637-100
Activin RIA/ALK‑2 in HUVEC Human Cells.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human Activin RIA/ALK-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 71412
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK‑2
Asp23-Val124
Accession # Q04771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Activin RIA/ALK-2 Fc Chimera (Catalog # 637-AR)
under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Activin RIA/ALK-2 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Activin RIA/ALK‑2 in HUVEC Human Cells. Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIA/ALK-2

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

References
  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
Long Name
Activin Receptor IA
Entrez Gene IDs
90 (Human); 11477 (Mouse)
Alternate Names
activin A receptor, type I; Activin RIA; ActivinRIA; ACTRI; ACVR1; ACVR1A; ACVRLK2; ALK2; ALK-2; FOP; SKR1; TSRI

Product Datasheets

Citations for Human Activin RIA/ALK-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils
    Authors: Y Kimishima, T Misaka, T Yokokawa, K Wada, K Ueda, K Sugimoto, K Minakawa, K Nakazato, T Ishida, M Oshima, S Koide, K Shide, K Shimoda, A Iwama, K Ikeda, Y Takeishi
    Nature Communications, 2021;12(1):6177.
    Species: Transgenic Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate
    Authors: HN Arai, F Sato, T Yamamoto, K Woltjen, H Kiyonari, Y Yoshimoto, C Shukunami, H Akiyama, R Kist, A Sehara-Fuj
    Cell Rep, 2019;29(3):603-616.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7.
    Authors: Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH
    Eur. J. Immunol., 2011;41(11):3135-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
    Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
    J. Biol. Chem., 2007;282(32):23337-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human Activin RIA/ALK-2 Antibody
By Anonymous on 08/27/2018
Application: WB Sample Tested: HAEC human aortic endothelial cells,HEK293T human embryonic kidney cell line,HUVEC human umbilical vein endothelial cells Species: Human

10ug of protein collected from untreated HEK, HUVEC, and HAEC cells was loaded into each well.

Blocked in Prometheus OneBlock Western-CL Blocking Buffer for 1hr @ room temp

Primary antibody: Anti-ALK2 MAB637, mouse, RnD 1:500 overnight @ 4C

Secondary antibody: goat-α-mouse HRP (Thermo) 1:10,000 1 hr @ room temp

Substrate: Luminata Forte Western HRP Substrate Millipore 2 min

Imaging: 300 sec exposure