Human Activin RIIA Antibody Summary
Accession # P27037
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Activin RIIA in Human Prostate Cancer Tissue. Activin RIIA was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Activin RIIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF340) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Activin RIIA
Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.
- Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
- Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.
Citations for Human Activin RIIA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Activin receptors regulate the oligodendrocyte lineage in health and disease
Authors: A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, TJ Yuen, GH Su, JC Becher, C Smith, A Williams, VE Miron
Acta Neuropathol., 2018;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
Treatment with a soluble receptor for activin improves bone mass and structure in the axial and appendicular skeleton of female cynomolgus macaques (Macaca fascicularis).
Authors: Fajardo RJ, Manoharan RK, Pearsall RS, Davies MV, Marvell T, Monnell TE, Ucran JA, Pearsall AE, Khanzode D, Kumar R, Underwood KW, Roberts B, Seehra J, Bouxsein ML
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Plasma
Applications: ELISA Development
Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
Authors: Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK
Dev. Biol., 2006;298(1):132-48.
Sample Types: Whole Tissue
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
Dev. Biol., 2006;295(2):473-85.
Sample Types: Tissue Homogenates
Applications: Western Blot
Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
Authors: Bristol SK, Woodruff TK
Biol. Reprod., 2004;70(3):846-59.
Sample Types: Whole Tissue
Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway.
Authors: Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H
J. Biol. Chem., 2002;277(21):19008-18.
Sample Types: Cell Lysates
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