Human ADAMTS5 Antibody Summary
Accession # Q9UNA0
Complete Your Research
Complete Your Experiment
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human ADAMTS5 DuoSet ELISA Kit (Catalog # DY2198-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human ADAMTS5 ELISA Standard Curve. Recombinant Human ADAMTS5 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB2198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as Aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-Macroglobulin (3), Aggrecan (4), and Brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified recombinant human (rh) ADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and rhTIMP-3 (R&D Systems, Catalog # 973-TM).
- Porter, S. et al. (2005) Biochem. J. 386:15.
- Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
- Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
- Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
- Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
- Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
- Glasson, S.S. et al. (2005) Nature, 434: 644.
- Stanton, H. et al. (2005) Nature, 434:648.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human ADAMTS5 Antibody
There are currently no reviews for this product. Be the first to review Human ADAMTS5 Antibody and earn rewards!
Have you used Human ADAMTS5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image