Human Adenosine A2aR/A2bR Antibody

Catalog # Availability Size / Price Qty
MAB94972-100
MAB94972-SP
Human Adenosine A2aR/A2bR Antibody in Flow Cytometry
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Human Adenosine A2aR/A2bR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 599743
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Adenosine A2aR  in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow  Cytometry. View Larger

Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosene A2aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Adenosine A2bR  in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow  Cytometry. View Larger

Detection of Adenosine A2bR in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Adenosene A2bR and eGFP was stained with (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR/A2bR

Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A1, A2A, A2B, and A3 have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A2A and A2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.

Long Name
Adenosine A2A Receptor/Adenosine A2B Receptor
Alternate Names
Adenosine A2aR/A2bR

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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