Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A1, A2A, A2B, and A3 have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A2A and A2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.
Human Adenosine A2aR/A2bR Antibody
R&D Systems | Catalog # MAB94972
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 599743
Loading...
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274
Met1-Ser412
Accession # P29274
Specificity
Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Adenosine A2aR/A2bR Antibody
Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Adenosene A2aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Detection of Adenosine A2bR in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Adenosene A2bR and eGFP was stained with (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Applications for Human Adenosine A2aR/A2bR Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2aR or Adenosine A2bR and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2aR or Adenosine A2bR and eGFP
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Adenosine A2aR/A2bR
Long Name
Adenosine A2A Receptor/Adenosine A2B Receptor
UniProt
Additional Adenosine A2aR/A2bR Products
Product Documents for Human Adenosine A2aR/A2bR Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Adenosine A2aR/A2bR Antibody
For research use only
Related Research Areas
Customer Reviews for Human Adenosine A2aR/A2bR Antibody
There are currently no reviews for this product. Be the first to review Human Adenosine A2aR/A2bR Antibody and earn rewards!
Have you used Human Adenosine A2aR/A2bR Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars