Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Adrenomedullin/ADM Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Adrenomedullin/ADM
Ala22-Gly147
Accession # P35318

Specificity

Detects human Adrenomedullin/ADM in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Adrenomedullin/ADM Antibody

Adrenomedullin/ADM antibody in Human Heart by Immunohistochemistry (IHC-P).

Adrenomedullin/ADM in Human Heart.

Adrenomedullin/ADM was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Adrenomedullin/ADM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6108) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Adrenomedullin/ADM Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Reviewed Applications

Read 1 review rated 5 using AF6108 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adrenomedullin/ADM

Adrenomedullin (ADM; also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The human ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-92), the ADM precursor (amidation is required for maturation) (aa 95‑147), and a C-terminal propeptide (aa 148-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 95‑146). Depending upon the tissue, truncated forms of ADM likely also occur, including variants spanning aa 120-146 and 128-146. Over aa 22-147, the human ADM proprecursor shares 70% aa identity with mouse ADM proprecursor.

Alternate Names

ADM

Entrez Gene IDs

133 (Human); 11535 (Mouse); 25026 (Rat)

Gene Symbol

ADM

UniProt

P35318 (Human)

Additional Adrenomedullin/ADM Products

Product Documents for Human Adrenomedullin/ADM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adrenomedullin/ADM Antibody

For research use only

Related Research Areas

Hypoxia Inducible Factors (HIFs)

Citations for Human Adrenomedullin/ADM Antibody

Customer Reviews for Human Adrenomedullin/ADM Antibody (1)

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  • Human Adrenomedullin/ADM Antibody
    Name: Ella Waters
    Application: MTS Assay
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 04/25/2018
    Greater concentrations of AM produced more cell proliferation, as expected. Inhibitors were then used to observe the effect of blocking VEGFA on AM.

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Protocols

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