Human AGTR-1 Antibody

Catalog # Availability Size / Price Qty
MAB10244-100
MAB10244-SP
Human AGTR-1 Antibody in Flow Cytometry
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Human AGTR-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human AGTR-1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1010103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human AGTR-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of AGTR-1  in HEK293 Human Cell Line Transfected with Human AGTR-1 and eGFP by Flow  Cytometry. View Larger

Detection of AGTR-1 in HEK293 Human Cell Line Transfected with Human AGTR-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human AGTR-1 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human AGTR-1 Monoclonal Antibody (Catalog # MAB10221) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AGTR-1

Type-1 angiotensin II receptor, AGTR1, is a seven transmembrane domain (7TM) G protein-coupled receptor (GPCR) that mediates the cardiovascular effects of angiotensin II. At least four transcript variants have been described for the AGTR1 gene. 

Long Name
Angiotensin II Receptor Type 1
Entrez Gene IDs
185 (Human); 11607 (Mouse); 24180 (Rat)
Alternate Names
AG2S; AGTR1; AGTR-1; AGTR1AAngiotensin II type-1 receptor; AGTR1B; Angiostensin Receptor; angiotensin II receptor, type 1; angiotensin receptor 1; angiotensin receptor 1B; AT1; AT1AR; AT1AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1AGTR1B; AT2R1BAT1BR; HAT1R; type-1 angiotensin II receptor; type-1B angiotensin II receptor

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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