Detects human ALCAM/CD166 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BCAM, recombinant mouse OCAM, rhMCAM, or rhEpCAM is observed.
Monoclonal Mouse IgG1 Clone # 105901
Protein A or G purified from ascites
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166 Trp28-Ala526 Accession # Q13740
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human ALCAM/CD166 by Western Blot. Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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CD318 is a ligand for CD6 Authors: G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Functional Assay
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