Human ALCAM/CD166 Antibody

Catalog # Availability Size / Price Qty
MAB656-SP
MAB656-100
MAB656-500
Detection of Human ALCAM/CD166 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human ALCAM/CD166 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALCAM/CD166 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BCAM, recombinant mouse OCAM, rhMCAM, or rhEpCAM is observed.
Source
Monoclonal Mouse IgG1 Clone # 105901
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Adhesion Blockade
The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human ALCAM/CD166 by Western Blot. View Larger

Detection of Human ALCAM/CD166 by Western Blot. Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

Citations for Human ALCAM/CD166 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD318 is a ligand for CD6
    Authors: G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  2. Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype
    Sci Rep, 2016;6(0):29712.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  3. Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS.
    Authors: Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S
    J. Neurosci., 2011;31(16):5942-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  4. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
    Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP
    Nat. Cell Biol., 2010;12(5):468-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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Reconstitution Buffers

Secondary Antibodies

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