Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha 2-Macroglobulin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived alpha 2-Macroglobulin

Specificity

Detects human alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha 2-Macroglobulin-like 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human alpha 2‑Macroglobulin Antibody

Detection of Human a2-Macroglobulin antibody by Western Blot.

Detection of Human alpha 2‑Macroglobulin by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human a2-Macroglobulin antibody by Simple WesternTM.

Detection of Human alpha 2‑Macroglobulin by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for a2-Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

a2-Macroglobulin antibody in human PBMCs by Immunocytochemistry (ICC).

alpha 2‑Macroglobulin in human PBMCs.

a2-Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human alpha 2‑Macroglobulin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Human alpha 2-Macroglobulin (Catalog # 1938-PI), see our available Western blot detection antibodies

Simple Western

1 µg/mL
Sample: Human serum

Western Blot

0.5 µg/mL
Sample: Human serum

Reviewed Applications

Read 4 reviews rated 4.3 using AF1938 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: alpha 2-Macroglobulin

Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

References

  1. Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
  2. Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Entrez Gene IDs

2 (Human)

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Human alpha 2‑Macroglobulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human alpha 2‑Macroglobulin Antibody

For research use only

Citations for Human alpha 2‑Macroglobulin Antibody

Customer Reviews for Human alpha 2‑Macroglobulin Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human alpha 2‑Macroglobulin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human alpha 2-Macroglobulin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human alpha 2‑Macroglobulin Antibody AF1938
  • Human alpha 2-Macroglobulin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2018
  • Human alpha 2-Macroglobulin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018
  • Human alpha 2-Macroglobulin Antibody
    Name: Carly Garrison
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies