Human alpha 2-Macroglobulin Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human alpha 2‑Macroglobulin by Western Blot.
Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human
alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).
A specific band was detected for
alpha 2‑Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha 2‑Macroglobulin in human PBMCs.
alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
alpha 2‑Macroglobulin in human PBMCs.
Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.
- Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
- Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.
Citations for Human alpha 2-Macroglobulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
EMBO Mol Med, 2014;6(1):27-42.
Sample Types: Whole Cells
2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter.
Authors: Kim JC, Kim JY, Yeom SR, Jeong BY, Hwang HZ, Park KJ, Lee SW
Sample Types: Serum
Applications: Western Blot
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