Detection of Human alpha -Internexin by Western Blot. Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti‑Human alpha -Internexin Monoclonal Antibody (Catalog # MAB4844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for alpha -Internexin at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
alpha -Internexin ( alpha -Inx; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -Inx is one of four Class IV neuronal IF proteins. It both self-assembles and complexes with NF-L, H and M in select cortical and cerebellar neurons. alpha -Inx contains one DNA-binding region (aa 10‑92), a poly‑Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -Inx shows more that 96% aa identity with mouse, canine and rat alpha -Inx.
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