Detection of Human alpha -Sarcoglycan by Western Blot.
Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human alpha -Sarcoglycan Monoclonal Antibody (Catalog # MAB7445) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha -Sarcoglycan at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Alpha-sarcoglycan is one of six known
sarcoglycans, which are type I ( alpha - and epsilon -) or type II ( beta -, gamma -, δ- or zeta -)
transmembrane glycoproteins. Sarcoglycans alpha and gamma are expressed
exclusively in the sarcoplasmic reticulum in skeletal and cardiac muscle, while
others are more widely expressed. Sarcoglycans form heterotetrameric,
pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts
laterally with the dystroglycan complex, and both complexes are components of
the dystrophin-associated glycoprotein complex (DGC), which forms a link
between the cytoskeleton and the extracellular matrix. Mutations in
sarcoglycans are associated with recessive autosomally inherited limb-girdle muscular
dystrophy. Human alpha -sarcoglycan
shares 90% amino acid sequence identity with mouse and rat alpha -sarcoglycan within the extracellular domain.
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