Human alpha -Sarcoglycan Antibody

Catalog # Availability Size / Price Qty
MAB7445-SP
MAB7445-100
Detection of Human  alpha -Sarcoglycan by Western Blot.
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Human alpha -Sarcoglycan Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Sarcoglycan in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 769109
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human alpha -Sarcoglycan
Gln24-Ala290 (predicted)
Accession # Q16586
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human  alpha -Sarcoglycan by Western Blot. View Larger

Detection of Human alpha -Sarcoglycan by Western Blot. Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human alpha -Sarcoglycan Monoclonal Antibody (Catalog # MAB7445) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha -Sarcoglycan at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Sarcoglycan

Alpha-sarcoglycan is one of six known sarcoglycans, which are type I ( alpha - and epsilon -) or type II ( beta -, gamma -, δ- or zeta -) transmembrane glycoproteins. Sarcoglycans alpha and gamma are expressed exclusively in the sarcoplasmic reticulum in skeletal and cardiac muscle, while others are more widely expressed. Sarcoglycans form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited limb-girdle muscular dystrophy. Human alpha -sarcoglycan shares 90% amino acid sequence identity with mouse and rat alpha -sarcoglycan within the extracellular domain.

Entrez Gene IDs
6442 (Human); 20391 (Mouse); 303468 (Rat)
Alternate Names
50-DAG; Adhalin; ADL; alphaSarcoglycan; alpha-Sarcoglycan; DAG2; DMDA2; DMDA250kD DAG; Dystroglycan-2; LGMD2D; LGMD2Dalpha (50kD dystrophin-associated glycoprotein); sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SCARMD1; SGCA

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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