Human alpha -Synuclein Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human alpha -Synuclein in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 970114
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human alpha -Synuclein
    Met1-Ala140
    Accession # P37840
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    5 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human alpha -Synuclein by Western Blot. Western blot shows lysates of human brain (motor cortex and cerebellum) tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB13383) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha -Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
alpha -Synuclein in Human Brain. alpha -Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB13383) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Synuclein
alpha -Synuclein is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a precursor of a minor peptide found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in alpha -Synuclein are associated with familial Parkinson's disease.
  • Entrez Gene IDs:
    6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)
  • Alternate Names:
    alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; PD1; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha
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