Human Androgen R/NR3C4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB58763-100
MAB58763-SP
Human Androgen R/NR3C4 Antibody in Data
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Human Androgen R/NR3C4 Antibody Summary

Species Reactivity
Human
Specificity
Detects Androgen R/NR3C4 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2621B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic Androgen R/NR3C4 peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
LNCaP human prostate cancer cell line
Immunohistochemistry
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human prostate cancer
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
LnCAP human prostate carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human Androgen R/NR3C4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Androgen R/NR3C4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry View Larger

Androgen R/NR3C4 in Human Prostate Cancer. Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry View Larger

Detection of Androgen R/NR3C4 in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate carcinoma cell line was stained with Rabbit anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763, filled histogram) or control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Androgen R/NR3C4

The Androgen Receptor (AR) is a 90 kDa steroid hormone receptor that is critical for the development and function of the male reproductive system. AR binding to testosterone or 5 alpha -dihydrotestosterone (DHT) triggers receptor dimerization followed by translocation to the nucleus where it promotes transcription of androgen responsive genes. Multiple polymorphisms in AR are linked to the development of prostate cancer. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Long Name
Androgen Receptor
Entrez Gene IDs
367 (Human)
Alternate Names
AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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