Human Androgen R/NR3C4 Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Androgen R/NR3C4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Androgen R/NR3C4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Androgen R/NR3C4 in Human Prostate Cancer. Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Androgen R/NR3C4 in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate carcinoma cell line was stained with Rabbit anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58763, filled histogram) or control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Androgen R/NR3C4
The Androgen Receptor (AR) is a 90 kDa steroid hormone receptor that is critical for the development and function of the male reproductive system. AR binding to testosterone or 5 alpha -dihydrotestosterone (DHT) triggers receptor dimerization followed by translocation to the nucleus where it promotes transcription of androgen responsive genes. Multiple polymorphisms in AR are linked to the development of prostate cancer. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.
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