Human ASGR2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human ASGR2 by Western Blot.
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Product Details
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Human ASGR2 Antibody Summary

Species Reactivity
Detects human ASGR2 in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2327C
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ASGR2
Accession # P07307
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ASGR2 antibody by Western Blot. View Larger

Detection of Human ASGR2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ASGR2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of ASGR2 antibody in HEK293 Human Cell Line Transfected with Human ASGR2 and eGFP antibody by Flow Cytometry. View Larger

Detection of ASGR2 in HEK293 Human Cell Line Transfected with Human ASGR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ASGR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry ASGR2 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

ASGR2 in Human Liver. ASGR2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR2) is a subunit of the Asialoglycoprotein receptor. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Expressed on hepatocytes, the Asialoglycoprotein receptor mediates endocytosis and lysosomal degradation of glycoproteins to mediate serum glycoprotein homeostasis. The ASGR receptor binds plasma glycoproteins which have had the terminal sialic acid residue removed. An alternatively spliced variant, H2, which can be shed, has been proposed as a marker for liver fibrosis.

Long Name
Asialoglycoprotein Receptor 2
Entrez Gene IDs
433 (Human); 11890 (Mouse); 29403 (Rat)
Alternate Names
ASGPR 2; ASGP-R 2; ASGPR2; ASGP-R2; ASGR2; asialoglycoprotein receptor 2; CLEC4H2; CLEC4H2FLJ60040; C-type lectin domain family 4 member H2; HBxAg-binding protein; HBXBP; Hepatic lectin H2; HL-2

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