Human ATM Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ATM in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ATM
    Arg2138-Arg2400
    Accession # Q13315
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human ATM.
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
ATM in Human Breast. ATM was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human ATM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2290) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATM

ATM (Ataxia Telangiectasia Mutated) is a 350-370 kDa member of the ATM subfamily, PI3/PI4-kinase family of enzymes. It is ubiquitously expressed, and serves as a DNA damage sensor. ATM is activated via autophosphorylation at double strand breaks. Following activation, multiple substrates are phosphorylated, including Chk2, and ATR is recruited and activated as part of an integrated repair circuit. Human ATM is 3056 amino acids (aa) in length. It contains one FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962) and a second, C-terminal FAT domain (aa 3024-3056). There are at least six Ser and four Thr utilized phosphorylation sites, and one critical acetylation activation site at Lys3016. There are at least four potential splice variants. One shows a Trp substitution for aa 536-3056, a second contains an eight aa substitution for aa 2506-3056, a third possesses a five aa substitution for aa 1637-3056, while a fourth contains a premature truncation after Lys2756. Over aa 2138-2400, human ATM shares 82% aa identity with mouse ATM.

  • Long Name:
    Ataxia Telangiectasia-mutated
  • Entrez Gene IDs:
    472 (Human); 11920 (Mouse)
  • Alternate Names:
    AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL
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